LOCUS       sequence-156968-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting human telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1651-sgTelomere(F+E)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(296..329)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     333..353
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg cgccatctct
      121 aggcccgcgc cggccccctc gcacggactt gtgggagaag ctcggctact cccctgcccc
      181 ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca
      241 gataatctgt tctttttaat actagctaca ttttacatga taggcttgga tttctataac
      301 ttcgtatagc atacattata cgaagttata aacagcacaa aaggaaactc accctaactg
      361 taaagtaatt gtgtgttttg agactataag tatcccttgg agaaccacct tgttggttag
      421 ggttagggtt agggttagtt taagagctat gctggaaaca gcatagcaag tttaaataag
      481 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctcgagtact
      541 aggatccatt aggcggccgc gtggataacc gtattaccgc catgcattag ttattaatag
      601 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      661 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      721 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      781 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      841 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      901 gactttccta c
//
