LOCUS       sequence-156979-        8348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary Expression vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Nowack MK, Shirzadi R, Dissmeyer N, Dolf A, Endl E, Grini PE, 
            Schnittger A
  TITLE     Bypassing genomic imprinting allows seed development.
  JOURNAL   Nature. 2007 May 17;447(7142):312-5. Epub 2007 Apr 29.
  PUBMED    17468744
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-05-17"; volume: "447(7142)"; pages: "312-5. Epub 2007 
            Apr 29"
COMMENT     Sequence Label: pAM-PAT-ProCDKA;1::CDKA;1:YFP
FEATURES             Location/Qualifiers
     source          1..8348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    386..410
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      522..1231
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1292..1396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1397..1465,1466..2257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1615..1634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2428..3016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2917..2936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3328..3352
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3446..3698)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3718..4269)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4311..4490)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    6712..6736
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7619..7621,7622..7624,7625..8338)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7664..7685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7925..7944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8272..8293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(join(8339..8348,1..15))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgtacaaa gtggtgatcc tccgttccat gggctagaag cttgttgaga attcatcgcc
       61 cgggactggg ttaacttccg atcgattcta gactagttct agagtccgca aaaatcacca
      121 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
      181 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
      241 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
      301 caaaatccag tgaccgggcg gccgccaccg cggtggaggg ggatcagatt gtcgtttccc
      361 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
      421 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
      481 catttgtatg tgtacatcac cgacgagcaa ggcaagaccg agcgcctttc cgacgctcac
      541 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
      601 aacgccgtcg aagccgtgtg cgagacaccg gccgccggcg ttgtggatac ctcgcggaaa
      661 acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg
      721 gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag
      781 cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa
      841 gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag
      901 gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga
      961 catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt
     1021 tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt
     1081 ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     1141 ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg
     1201 gccgcgaacg gcctcacccc aaaaatggca gcgctggcag tccttgccat tgccgggatc
     1261 ggggcagtaa cgggatgggc gatcagcccg acaagctacc cctatttgtt tatttttcta
     1321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1621 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3061 gccttttgct cacatggact ctagctagag gatcacaggc agcaacgctc tgtcatcgtt
     3121 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     3181 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     3241 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     3301 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     3361 gcttagacaa cttaataaca cattgcggac gtttttaatg atcgaatact aacgtctcta
     3421 ccagatatca gcttgcatgc cggtcgatct agtaacatag atgacaccgc gcgcgataat
     3481 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     3541 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     3601 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     3661 aatcttaaga aactttattg ccaaatgttt gaacgatctg cttgactcta ggggtcatca
     3721 gatttcggtg acgggcagga ccggacgggg cggcaccggc aggctgaagt ccagctgcca
     3781 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg
     3841 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc
     3901 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga
     3961 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc
     4021 gtaggcgttg cgtgccttcc agggacccgc gtaggcgatg ccggcgacct cgccgtccac
     4081 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg
     4141 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat
     4201 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc
     4261 tgggctcatg gtagatcccc tcgatcgagt tgagagtgaa tatgagactc taattggata
     4321 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
     4381 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
     4441 attaaactcc agaaacccgc ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc
     4501 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
     4561 tccgctcatg atcagattgt cgtttcccgc cttcggtttg ggcgcgccgg gaagatagaa
     4621 gggaagagag aggtagacgg aaagagaaat ggagaaaaat agataaaggg gtaaacttgg
     4681 aattaatata caaaatatag aggatatcag ttctttttcc ttttgatgtt agtttcttaa
     4741 ttataaactt tttgttggtt agttttgcca attcccttac ttacaactga taaccgtata
     4801 gctcttttat cagaaaaaac tttatatatc taatctccca aaatatacaa atgtgagaaa
     4861 tatctgaaaa taaatcaatt ctcaccaatt atatatattt gattacttaa aaatgttaat
     4921 atatggcagt tgcttgttca gagaataact taaaaaattg atcacgagat cggcttatgt
     4981 ttaatatttt tgaactcttg ggataaaagt tttgaagata tatatatcgt agaaaagttt
     5041 tgtgttcttt aattagttac aaaattttga gttttttttt tttctttcat aaagtatcag
     5101 aatatcaggg tgatttattg aatagtttat tttttctcgt tctactcacg ttattcttgc
     5161 aagtattaat tttggtttgt ttataaaaaa agtatttgga aattttatgt gagcaagtgc
     5221 gaaataaacg aggattttgg gaagtatttt tttttttgta aaatcggtta tattgaacgt
     5281 tatactatat atattattat ataaatataa ctgctaaaga ctaaatttag atactaaaat
     5341 cagcattgat gaagatccac atgaataaga ctcgtctctt aactcaacat ttaacatatg
     5401 attttcatat tattgatcat ttatctttgt gttggtccaa ggtgccagac tttattattt
     5461 acattaaatg ttgagttgtt tactgacata ttttttaatt aaaacaactt tcatattatt
     5521 gcccatttat attttggttg gtcctccaaa gacttacagg atacatatgt ttctccattt
     5581 tcatttttat gatctaaaac gtctttttct aacttagatt ctaaatggtt acagcaaacc
     5641 ggacatacaa ttttgcttgt caaatggatc gttgagatag acaacaatcc aatgtaaaac
     5701 caaaactctt gatttataat tggtctgcaa tgattttttt ttggttcata taccatttta
     5761 taagatagca aaccattttc atcataaaag atatattttt ttatcattga ataccaatca
     5821 aacccggtat aataaataaa attgtaccaa attaatgcgg ttaggtaaat tcaatttatt
     5881 ggaaattaaa gaagagaaaa aaaaaataac gaacaaaatt tccttgacac aaaaaaaaaa
     5941 aaagaagaaa gaaaaaaccc ttactctcaa ttagtcaatc cccttcccta gtcctcctct
     6001 cttcaagctt ttcacagaaa accaccaccc ttctctctct actgcctttt taccacagag
     6061 aagagagagg atccgtcggt gtgctagtct cactgacact acatccgatc gtcgccggta
     6121 agttgatttc gttactctcc gatcaatttc ttttccggag gaggaacttg ttttccgtgc
     6181 gaaattctga cttgaacttt gttgtcaatt catcaaacgc tttatctttt ctccatcttc
     6241 aattatctta ctgcttcgtc gctgatctct acttgttttc ctccgttttg cattttcagt
     6301 ggcgtttctt agtgtttcac tagatttaga ctggttttgc ttgtgtgcat tttgtcatca
     6361 gtgtttatga ttgttcgctt tagctctatg tttctatgga tttgataata tatgagttcg
     6421 ctgctctgga ttcagctggc ttgtttgatt gttcattttc ataattctgt ccaaagaatc
     6481 aaatccacag tagctcggat ctgagattga tttgtttctt tgcgtctttg tgtagatttg
     6541 actttaattt tgagattgtt gtgtgctgat atacagctta attaatatag cctctgatgt
     6601 gttattatcg atgtgtcatt atcaatctac aggtgacatt ttataagtgt ggagtttact
     6661 tcagctttat tattcaggaa ttgctcgaga aagaggatcc acctgaggat cacaagtttg
     6721 tacaaaaaag caggctatgg atcagtacga gaaagttgag aagattggtg aaggaactta
     6781 cggtgtggtt tataaggcac gtgacaaagt gactaatgag acaattgctt tgaagaagat
     6841 caggctagag caggaggatg aaggtgttcc tagcacagca atcagagaaa tctccctctt
     6901 gaaagaaatg cagcatagca acattgtcaa attgcaggat gtggtgcaca gcgagaaacg
     6961 tttgtatctg gtttttgagt atcttgactt ggatctcaaa aagcacatgg attctactcc
     7021 tgatttctcc aaggatctac atatgatcaa aacatatctt taccagattc tccgtggaat
     7081 tgcgtattgc cactctcata gggttctcca tcgtgatctg aagccacaga atttgttgat
     7141 tgatcgccgc acaaactcac tgaagcttgc tgattttgga ctggccagag cattcggtat
     7201 ccctgtcagg acatttactc atgaggttgt tactctctgg taccgagcac cagagatact
     7261 cctaggatct catcattact ctacacctgt tgatatttgg tctgtggggt gcatatttgc
     7321 tgagatgatc agccaaaagc ccttatttcc tggagactcc gagattgatc aactcttcaa
     7381 gattttcaga atcatgggaa ctccgtacga ggatacatgg cgtggggtaa cttctctacc
     7441 ggattataaa tctgctttcc ctaaatggaa accaacggac ctagaaactt ttgtccccaa
     7501 tctagatccc gatggagtcg atctcctttc taaaatgctg ttaatggatc cgaccaaaag
     7561 aatcaacgca agagccgccc tggagcatga atacttcaag gatcttggag gcatgcctat
     7621 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     7681 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     7741 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     7801 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     7861 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     7921 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     7981 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     8041 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     8101 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     8161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     8221 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     8281 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac
     8341 ccagcttt
//
