LOCUS       sequence-156991-        4843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1927
FEATURES             Location/Qualifiers
     source          1..4843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3844..3861)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3844..3860)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3959..3976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4130..4718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4210..4229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4842..4843,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg ctgaaacgcg tcagactggg aattgccgtg
     1261 gctatgcccg aagtttggaa agtctctctc ggtctctttc tgggacagcc tttctttttc
     1321 cgggaagctt cctgtgccta gaggtggggc tctgtagctg gaggggtaga tggaacttgc
     1381 gaagcaaagg aaggcaataa aaggaagtta ggagaaaccg gaaacgtggg ccaggccttg
     1441 ctctgttgga gaagcggtgt ccccgcaaag tccctgcttg cgctggtccc ggccggagcc
     1501 cagatccccc aaacgcctgt cacttctcat cgagttgagg ctccggcccc cacccgcgcc
     1561 ttggtgaggg ccccccctca gccccaagcc ttcggctacc ctgaaaacgc ggctcccctc
     1621 gaaggggaaa caagtgcagt tcaatctcgt ctgagtgatc tacaaataag gacggaaagg
     1681 gtcgttttat cacggtcccg tttgtcgtga cgatgcaatt tcccggagcg gagcactgtc
     1741 acaaagtgac aaggctgcca caagcgcccc gactgatctt ttcaattagc cttccatgca
     1801 tgatccggag cgacttccgc ctatttccag aaattaagct caaacttgac gtgcagctag
     1861 ttttatttta aagacaaatg tcagagaggc tcatcatatt ttcccccctc ttctatattt
     1921 ggagcttatt tattgctaag aagctcaggc tcctggcgtc aatttatcag taggctccaa
     1981 ggagaagaga ggagaggaga ggagagctga acagggagcc acgtcttttc ctgggagggc
     2041 tgctatctaa gtcggggctg caggttggag atttttaagg aagtggaaat tggcaattgg
     2101 ctttgtgtgt ctgtggtttt tggggagggg gactacaaag aggggctaac tccctctccc
     2161 tattctctaa ggttggacca cagggatgag gttgtgagat acaaagataa aggagggatg
     2221 gggaacacta tgatgtggta tttttctttt ctgttttttc tttttgataa tatctatcct
     2281 tggctaaggg gagggcggag ttcagcggcg gaaataaagc gagcagtggc tggtgcgaac
     2341 cgactcccgg ctctaagcct tacttgcggt ggccggactt gtctgtggct gaagccgagc
     2401 ccgggctctg actctcacgt ctgcactgga ggtgcggaaa cctggactgg ggttcaccag
     2461 ccacatactg gctgctctgg ttgttctctc ctcttccttc ttctattcta accaaacaaa
     2521 acccaactcg atgcttgtgc caggccttgg ccagtttgga cggtgatgga aacatttctg
     2581 gattttagcg tctaagggag cactcaaggg ctgtaaggtt tgctatcact gtcccaacac
     2641 tgcagagacc ttgaaggttc agtgtgggat ctgtagaacc catggtttgc ggtgacactc
     2701 agaggggatc tttgggagat ttatagagcc ggttcttagc gctttgtggg ttcagaggct
     2761 ggctgcagtg tttatgaaga ggggcagtgg gctggggaca ctgctggggt tatggctgta
     2821 gtgaggtcca tgtgtacttg ttccttggca tgtgtctgac tctgtgttgc tgctgcagta
     2881 cagtgggcag gggcacggtt gcttggactg ggctgcccga tcttggggat cggaggaggg
     2941 gacagggtga ttacccagag aggtagctgg ccagcctaag ggcagagatc ttggggccct
     3001 agtgcccgaa ggtgcggagg agcgcactcg gcaagactag tttcctgggg atcgactcta
     3061 cgccatacag gacggcggcc caggctggac cgggccgggc tagagcagtc acaggccggg
     3121 ccaaggaagg ccaaagcagg ggttggagcc ggccggaccc tgggtgggga gaagcaggct
     3181 cccgcccggc cggaaaacta gtcggcgcag agctgtgccc aactctagcc gccatgacgt
     3241 cacgcgggcc gggcagccaa tgaggacggc gctggcgtgg atattaagga aagttagcgc
     3301 ctgcctgagc accctctttt cttatcattg acatttaaac tctggggcag gtcctcgcgt
     3361 agaacgcggc tgtcaggcgc gcccgtcaaa agggcgacac aaaatttatt ctaaatgcat
     3421 aataaatact gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat
     3481 aattttgata tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc
     3541 tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt
     3601 aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt
     3661 tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat
     3721 attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt
     3781 ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt
     3841 aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg
     3901 tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct
     3961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct
     4561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4741 cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat
     4801 gagcttgcgc cgtcccgtca agtcagcgta atgctctgct ttt
//
