LOCUS       sequence-156994-        4756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple255 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1928
FEATURES             Location/Qualifiers
     source          1..4756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3757..3774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3757..3773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3872..3889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4043..4631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4123..4142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4755..4756,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttga cgggcgcgcc tgacagccgc gttctacgcg
     1261 aggacctgcc ccagagttta aatgtcaatg ataagaaaag agggtgctca ggcaggcgct
     1321 aactttcctt aatatccacg ccagcgccgt cctcattggc tgcccggccc gcgtgacgtc
     1381 atggcggcta gagttgggca cagctctgcg ccgactagtt ttccggccgg gcgggagcct
     1441 gcttctcccc acccagggtc cggccggctc caacccctgc tttggccttc cttggcccgg
     1501 cctgtgactg ctctagcccg gcccggtcca gcctgggccg ccgtcctgta tggcgtagag
     1561 tcgatcccca ggaaactagt cttgccgagt gcgctcctcc gcaccttcgg gcactagggc
     1621 cccaagatct ctgcccttag gctggccagc tacctctctg ggtaatcacc ctgtcccctc
     1681 ctccgatccc caagaatcaa agttgaacat aggcatctat tcatttagat taaatcagtt
     1741 gtcaaaaata aacccaaatt aacatttttt aacaatggaa atctaatttt ttacatgtgt
     1801 acattttgat atattcatta tattatcatt ctcatgttta tgcttttatt ctcaagtgat
     1861 atcagcatat aatagcatta cataggtttc aaagaatcat cagaagttgg tttaatactt
     1921 actacacttt atatatttca catgccatag ttaacctatc aaaagtgcat accaccttaa
     1981 ggagaacaca gttttgagaa tctgtctaat cctatttctt cccactttgg aatcttaatt
     2041 agaagagata agaaaaaaaa aaacatcatt taactaaatt attgtcttga atctgtagca
     2101 aaaggtataa tccgcaattg ggccaattag tgaaccccat agttagctat gctgcagcaa
     2161 acaatttgat tagatctgac accatcctag ttcccactgt tgttttgcca ggtatgatgg
     2221 tcagacttca aagagcttga ggcatcagca gggcaaggtt tggaggccag cttaggtccg
     2281 gttgttaatc tctctgctaa aaccacagca gctctcacac aaaacaagct tacacgacac
     2341 aatcatgcgg tattaaagtt agggttgagt catgccaatt tccctttgat tttttgatga
     2401 ttgaccaaag atggctacat gactagacac atgctaaaag tatcctgatt aggtaagttc
     2461 ccaaaatatt tgaagtattt tttaacttcc tatataaatg ttgtgaaagc ttattccctt
     2521 tgatcaaacc ttccaaagta atagtactgt actggggaaa aatgagcatt tcttaccggc
     2581 aacctgaata tcaaactttt cacttacctc tttatataaa tttgtcttta tattaataat
     2641 tccttaagga gaatatataa ggaaattgtt tctaagtggt aagcttttga aaaatctatt
     2701 aagcactttg gaagaaaaaa catttttccc tgagtcattt atgttcactg acagtttttc
     2761 tgaaatttct ttataattgt tttccactta aatgttgagt aaagtgccct tccaagatgt
     2821 ggttgcataa gtgttctttt ctctgcattc tgatttgttg aaatattgca gtaatagtga
     2881 catgtttcca ttctgagaaa agcctaaggc tgctttagta ttattccaaa tgattgaaac
     2941 tgccatcttg ctgactttcc cttaggcagt gtaagtcctg tttgcttctg tgttcatttc
     3001 acatgcttcc tgttagtttg gctggcagaa catcattgct tcatttggca aggaagagta
     3061 gattcatatt tggctattat atgtcttaaa gtttctgtgt ggtcatcaac acgtaggata
     3121 tgaaacttat aattagttat atcaaatgaa tcatctttct ttgagaaata taattaaaat
     3181 atgacacctt gaccgtaatt tttatataaa aatcttcatt ttataaattg aacttagggt
     3241 ctaatatttt tacattgcac tatttattta ttcataacta agttactcaa cgcgtttcag
     3301 cgaagggcga cacaaaattt attctaaatg cataataaat actgataaca tcttatagtt
     3361 tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa ctgattttcc
     3421 ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa atattgtata
     3481 tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc aatcgaaaaa
     3541 gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta aataattctc
     3601 atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct ttccctaaac
     3661 tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat taacgattac
     3721 tcgttatcag aaccgcccag ggggcccgag cttaagactg gccgtcgttt tacaacacag
     3781 aaagagtttg tagaaacgca aaaaggccat ccgtcagggg ccttctgctt agtttgatgc
     3841 ctggcagttc cctactctcg ccttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4441 cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta tttggtatct
     4501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg
     4681 cgcgcgtaac tcacgttaag ggattttggt catgagcttg cgccgtcccg tcaagtcagc
     4741 gtaatgctct gctttt
//
