LOCUS       sequence-156995-        4785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple256 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1929
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3786..3803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3786..3802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3901..3918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4072..4660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4152..4171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4784..4785,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctgacagcc gcgttctacg
     1261 cgaggacctg ccccagagtt taaatgtcaa tgataagaaa agagggtgct caggcaggcg
     1321 ctaactttcc ttaatatcca cgccagcgcc gtcctcattg gctgcccggc ccgcgtgacg
     1381 tcatggcggc tagagttggg cacagctctg cgccgactag ttttccggcc gggcgggagc
     1441 ctgcttctcc ccacccaggg tccggccggc tccaacccct gctttggcct tccttggccc
     1501 ggcctgtgac tgctctagcc cggcccggtc cagcctgggc cgccgtcctg tatggcgtag
     1561 agtcgatccc caggaaacta gtcttgccga gtgcgctcct ccgcaccttc gggcactagg
     1621 gccccaagat ctctgccctt aggctggcca gctacctctc tgggtaatca ccctgtcccc
     1681 tcctccgatc cccaagacgc accggggaga gcccggagtg gaggctctgg gctcagccct
     1741 cccaggccta ggccaccgtg ccctgagtcc ctccccaaga cttctcggac taagtgggcc
     1801 agactcttgt caggggaggg atcaccagga cctcagattg ctgggggcgg aggggtggac
     1861 gctcgtgagt gcgaagttcg ggtgtcactt gggtgtgtcc agtgtctaca agtgggaatc
     1921 ctacggtttc aaaagtcagg cagggatggg gttgcctaca ctcggtgggg tcaatcgcta
     1981 gtctcaccag aggcgggagc gggcctgggg gccctctgag ctcgcggagg cggcgggttc
     2041 ggggcccgtg cgggcggggt cgcccagggg agcccgggcc ccgcgcgtca ggaggcctcc
     2101 gccggccgag cggccgcgct gggaaacaag aagcgagcgc tttgctccct atcttcccag
     2161 tgtccgtcct atattgtttt ctgctcttaa aactgacatg tctaattggc aattggtgcc
     2221 gaatcgtgtc cagaggtctc ttgtggaaca tttctacagc tgtctccttc aactagacgc
     2281 ttattcatgt cgccttaatg agaaacaaaa cgttctctaa tgagcaatta cagagcgaca
     2341 ggattgttcc cataacaaat tcttgcgagt gacagaagca tcctttgtac cagatatttc
     2401 gcatactgtt accgattttc ccttccctcg cccgactcca tggaggcgcc gggcatccgg
     2461 agccggtccg agagctcacg gatgcgccgg cagcctcctc gcagtcccgc gcgccgcccc
     2521 tggccccgag cccctcggct ccgttgtgct cctgggagtg agacgccacg tcccattatc
     2581 ccgcatatta tctccttgtc acgaggacaa caaataccga ttaatcttcg gagtaaactg
     2641 tttcctattc ttgaccaagc tacctcgggg tgtggagggg agcggagggg agccaactga
     2701 ccctcccctc cgccccaccc cccccatccc ccgctcttct tcctccagcc cttctcacag
     2761 aacctggggt gtttcaggtc ccatctctcc tcccagacca gagcggctcc cggcatcccg
     2821 ccgtcctgct cacagtatct tcacctcacc acttccacat agtcgccacc cacagagagt
     2881 gagaaagtgg gacccagagg ggcgaacggg ggaggggcaa ggatagtggg gtggttttag
     2941 ttgggagacc accacttcca atgccccatc cccgactcgc cctgcatctc cctgctcacc
     3001 ttttcatgtg caccctactc atagtcacat aatttgtcaa ttacagcaaa aagcaacagg
     3061 atacaattat gttaacctcg cagtctgtca gtgcgttatc ttgtcacact tctacaaggg
     3121 ggctctccct gcattctaat gtcaaatttg aatagtgcac cacgcagctc gttcttatgc
     3181 acacagtgct gcccacaaac acacacacgc acccaccagc caccttcata ccgctcctga
     3241 catacacaac cctcacattc ccaggccaac aaaatggttc aacaaacacc acctacatac
     3301 aatgtggtcg atgtgtccac gcgtttcagc gaagggcgac acaaaattta ttctaaatgc
     3361 ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt
     3421 ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat
     3481 tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt
     3541 ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt
     3601 tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa
     3661 atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt
     3721 ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc
     3781 ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc
     3841 cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc
     3901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg
     4501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4681 tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc
     4741 atgagcttgc gccgtcccgt caagtcagcg taatgctctg ctttt
//
