LOCUS       sequence-156998-        4855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1930
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3856..3873)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3856..3872)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3971..3988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4142..4730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4222..4241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4854..4855,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctgacagcc gcgttctacg
     1261 cgaggacctg ccccagagtt taaatgtcaa tgataagaaa agagggtgct caggcaggcg
     1321 ctaactttcc ttaatatcca cgccagcgcc gtcctcattg gctgcccggc ccgcgtgacg
     1381 tcatggcggc tagagttggg cacagctctg cgccgactag ttttccggcc gggcgggagc
     1441 ctgcttctcc ccacccaggg tccggccggc tccaacccct gctttggcct tccttggccc
     1501 ggcctgtgac tgctctagcc cggcccggtc cagcctgggc cgccgtcctg tatggcgtag
     1561 agtcgatccc caggaaacta gtcttgccga gtgcgctcct ccgcaccttc gggcactagg
     1621 gccccaagat ctctgccctt aggctggcca gctacctctc tgggtaatca ccctgtcccc
     1681 tcctccgatc cccaagacgg agcctggaag aaagggaggg aggaaaaggg aggaaaaaaa
     1741 acaagttaca tggaattagt tggaagcggg cgcattcccc agcgaccgca gccattaaca
     1801 ttataatctc aaacgccatt ttttatgtaa cactattgtt agtaaatcaa ctcctcaggt
     1861 cctcagagac gctgacccct ggcaattcag tgaaagtgta attatctccc cgaatcttgg
     1921 acagatttaa gagattacat ttcaactaaa tctatatcaa tgctaattcc ctctctcccc
     1981 ccagcctgca ggtgctaggc acgggaaatg gcgtcaggcc ccagcagaac gcttcacaaa
     2041 gaggagaggg ctagctgtgt acagggccaa aagaaatgaa gccccacata aaattacacc
     2101 tttttttttt tgctacaaaa cctggttctt atatagcaaa gttaaacaaa gcagctatgc
     2161 taaattagcg ttacataaag gcgagtttaa tgctcggtta cagtcaactg gtgtatctct
     2221 gatggctaat tgtctatcaa aattaaagag aaaagaacaa atgattcgtt ataagaaggt
     2281 gttaaaagcc tcaacaaaag gacatttcac aaatataata tcggcagctc ccggattgcc
     2341 tgcggggttg ttcaggcccc gggcctgcac agctatgaga agtggggtgc agggtgctat
     2401 ttgcccagca aaagctcatt tcctcccaaa tgagtataat gaaaacccga gaggcgatat
     2461 tagtgtaaag atcgggatta gtccaaatag gagccgcgga cttgggcttc aaaggcgacc
     2521 cctactcttt ttgcccagtc tgggggcgga gggcgctcga agggatactt caaggacacc
     2581 acatccaact tgtcgcagcc ccacgttcag gcacaggagg gagcgaacat tgggcagagg
     2641 gtaatttcaa caaaggacgg caggctccgg ggatcccggc ttttccctga gttctctggg
     2701 ttggaaggag agggtccggg ctctgtcgga ggagtgggta cagcccagca catgcttttc
     2761 tgacgcgcag cgctggcctg gggagtgggt gcagctgctg cagccagccc ccaccccgcc
     2821 ccaggctggg aaggacgcag agtataatac gacggccccc ttccccaagg ggacactaga
     2881 tccagagctc caggcatatt tgttgttcaa agttaattat acctgaaagc aaccaaaatg
     2941 cgcacgccag gcccacggag gccgattgcc gaccagcgta gccggcccgc agcgccagag
     3001 aagccgctca ctccggcgga gtctctgcaa gagaatctga ggctggggaa taaaagccga
     3061 aactaaatca ggcagcaaga gtaacaaacc atctggaaaa tggacccacg gagccgcaag
     3121 aatgtacttt ttttttttat ccttgacaac ttgtgtccta aagaaagtgt attgaagata
     3181 attgaaatga ttacaattca ttgctgcctg actttgaata tgaacagtca cataatgaca
     3241 tccccgccac acaacaacag tttaatttcc attatccgac ttcgggatcc tagcgccagc
     3301 cgccggggca acgtggcgct aatgcagaaa tcttggctcc tgcctgcatt attcatttcg
     3361 gggacgtggg gatgggatag gagtggagac gcgtttcagc gaagggcgac acaaaattta
     3421 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc
     3481 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta
     3541 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata
     3601 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
     3661 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
     3721 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc
     3781 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg
     3841 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa
     3901 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc
     3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg
     4561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4741 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg
     4801 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg ctttt
//
