LOCUS       sequence-156999-        7421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Pesch M, Schultheiss I, Digiuni S, Uhrig JF, Hulskamp M
  TITLE     Mutual control of intracellular localisation of the patterning 
            proteins AtMYC1, GL1 and TRY/CPC in Arabidopsis.
  JOURNAL   Development. 2013 Aug;140(16):3456-67. doi: 10.1242/dev.094698.
  PUBMED    23900543
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-08"; volume: "140"; issue: 
            "16"; pages: "3456-67"
COMMENT     Sequence Label: pAM PAT-ProCPC GW
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..26
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(120..372)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(392..943)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(985..1164)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    2406..2530
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2555..2560,2561..2578,2579..2585)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2639..3298
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2705..2724)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3640..3945
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3824..3843
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3986..4110)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4481..4505
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4617..5326
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5387..5491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5492..5560,5561..6352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5710..5729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6523..7111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7012..7031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
       61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
      121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
      421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
      481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
      541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
      601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
      661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
      721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
      781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
      841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
      901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
      961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
     1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
     1261 ccgccttcgg tttgggcgcg cccagaaagc tttgtataca acaaatgtgc ttctttgctc
     1321 tttcgtctca ctccatttag tcaaaaattc catttgtgat ataaaaatga atagatgaaa
     1381 ccttgaaata tgtaatgaaa ccggtctcga gatgtggtta aagcatattt gtaatcaaaa
     1441 atatataatt aatgaagaaa aacaagtacg ttttcggacc acatcggacc agtagaggca
     1501 cacaaccata cgggtgttgt gcccactttt gacgacatta ccatcagacc aaaggaatat
     1561 atgaaagata catagagaga gatatgttct cgaactaact atttcttttt ttaccctctt
     1621 ctgattataa tattattttc ccgttttgct gttctcttct accaaacttt cctcttatat
     1681 caaactttca aatcgaattc ctgaacttta tatcacagtc cttatttgtt ttctcttgca
     1741 acaaatgaat ttttttgtta ttgttgaata gttatatttt taaataagta gctttgacag
     1801 aatgtatatt taatgtagaa tagcaaataa actacaaaca agaacacata gaagggacat
     1861 actggtcatt tcgaaagtca aagccagagg agaagctact atttctccaa ctgtctgtaa
     1921 acgaggaatt tttacaaccg caagtcaaag gatttaaaat aagtagttat ggttgtatct
     1981 gtcgttcttc tttctctatg aactaaaggc cgttggaagc aacacacaca cacacacagt
     2041 gaaacactac agtttacaga ttcgttttct ttagctcaat cgaattatat tatttcttaa
     2101 tcagtcaata tttaaataat ggaggatgat gataagtatt aaataaataa ttattctagg
     2161 attaatcaga catgcgcagt tatataataa tcagtttgat tttcttttcc ttttcgagcc
     2221 cctctctctc tcactctttt cttttccgag aacccaacaa aaaaaaagct actattaatc
     2281 cttcccctcg tgaggaaatc atttcttctt gtttctcgag atttattctc tttctctctc
     2341 tctttctctg tgtgtttcgt gtcttcagat tagttcggtc gagaaagagg atccacctga
     2401 ggatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
     2461 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     2521 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2581 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
     2641 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     2701 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     2761 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     2821 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     2881 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     2941 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     3001 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     3061 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     3121 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     3181 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt
     3241 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac
     3301 gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     3361 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg
     3421 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     3481 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     3541 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     3601 tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc
     3661 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     3721 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     3781 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     3841 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     3901 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     3961 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     4021 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     4081 ttctcgttca gctttcttgt acaaagtggt gatcctccgt tccatgggct agaagcttgt
     4141 tgagaattca tcgcccggga ctgggttaac ttccgatcga ttctagacta gttctagagt
     4201 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     4261 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     4321 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     4381 tctaattcct aaaaccaaaa tccagtgacc gggcggccgc caccgcggtg gagggggatc
     4441 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     4501 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     4561 gtttatccgt tcgtccattt gtatgtgtac atcaccgacg agcaaggcaa gaccgagcgc
     4621 ctttccgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc
     4681 tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccggccgc cggcgttgtg
     4741 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
     4801 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
     4861 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
     4921 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
     4981 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
     5041 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
     5101 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
     5161 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
     5221 ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag
     5281 gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt
     5341 gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgacaag ctacccctat
     5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat ggactctagc tagaggatca caggcagcaa
     7201 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     7261 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     7321 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     7381 ttttgtgccg agctgccggt cggggagctg ttggctggct g
//
