LOCUS       sequence-157-exp        4688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3522
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1059..1087)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1835..1855
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2433..2537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2538..2606,2607..3398)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2756..2775)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3569..4157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4058..4077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4311..4328
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4445..4466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4481..4486,4487..4504,4505..4511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4519..4535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4524..4546
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4543..4559
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4543..4559
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcgccaa atgtaaacct
       61 gtcatttctg tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg
      121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat
      181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc
      241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt
      301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc
      361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt
      421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa
      481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc
      541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc
      601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc
      661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa
      721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg
      781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag
      841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagcaacat ccgcgagatc
      901 gtggatccta tcgattcgcg gccgctgtac accgggattg gccaaaggac ccaaaggtat
      961 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg
     1021 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1081 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1141 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
     1201 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
     1261 tcactactct cacttatggt gttcaatgct tctcgagata cccagatcat atgaaacagc
     1321 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1381 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
     1441 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1501 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1561 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
     1621 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa
     1681 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1741 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1801 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1861 ctgggattac acatggcatg gacgaactat acaaaagccc acaagctttg gaattcgcta
     1921 gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     1981 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     2041 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     2101 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     2161 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     2221 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     2281 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa
     2341 attaggcgaa cgatgagaat tgtcctcgct tgggcccaaa aggccttttt tttttggcgc
     2401 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2521 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2581 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2641 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2701 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2761 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2821 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2881 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2941 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3001 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3061 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3121 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3181 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3241 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3301 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3361 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3421 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3481 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3961 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4201 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4261 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4321 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4381 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4441 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4501 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4561 atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa
     4621 ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga
     4681 aatgaaat
//
