LOCUS       sequence-157000-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple258 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1931
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3809..3826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3809..3825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3924..3941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4095..4683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4175..4194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4807..4808,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctgacagcc gcgttctacg
     1261 cgaggacctg ccccagagtt taaatgtcaa tgataagaaa agagggtgct caggcaggcg
     1321 ctaactttcc ttaatatcca cgccagcgcc gtcctcattg gctgcccggc ccgcgtgacg
     1381 tcatggcggc tagagttggg cacagctctg cgccgactag ttttccggcc gggcgggagc
     1441 ctgcttctcc ccacccaggg tccggccggc tccaacccct gctttggcct tccttggccc
     1501 ggcctgtgac tgctctagcc cggcccggtc cagcctgggc cgccgtcctg tatggcgtag
     1561 agtcgatccc caggaaacta gtcttgccga gtgcgctcct ccgcaccttc gggcactagg
     1621 gccccaagat ctctgccctt aggctggcca gctacctctc tgggtaatca ccctgtcccc
     1681 tcctccgatc cccaagtaag attaaatgaa agaagtaaat cctctttgaa ataagcaaat
     1741 gtcaggttga ccatttaaat acacagtact ttgcttatgt actatcagaa tggaaattta
     1801 tctcatcttt aaaccttgca atttctgtta ttcatataat tgagaaagtc ctagtttata
     1861 tcactgaaat cttgaataga tgagttctaa caaaaattga cttgtatcat tttagtttca
     1921 gatatttaga taaaaaggct tagtctttta tttatagata aaaggaaatc tgaccttttg
     1981 attaagaaat attgtcagta gcctttataa atgcttgttc atctgagggt ccatgacagg
     2041 agtactcttc ccagttgagt ttatcctaaa aatgaaatga taagagcctg ctggttatta
     2101 tccatgattc ttcttaatgg gggagccaca tggttattct tcatctccca cttgttctca
     2161 ccctttacta ccaacccagc cacaccccac ccccacccca acattgttca gtactcagca
     2221 caatgaatca ttttcaagtt gactcattgt gaactgtatc ccagctgttg gaatgtctgg
     2281 cagacatttt tgtttgggtg aagaataact ggctgaaacc caacctggat gagtttgata
     2341 taatggtctt tggctaaaga aatattgaga ggaacatgtt tatggcctct gaagcccctg
     2401 ttattcctgg agtaggacca tcccctgtgg cacagaactg aaagtaagga atcaatctgg
     2461 attattttct caccattgaa tgaaaaattg ccttagaaaa aataggcatt tccaaactat
     2521 gtttttctat acttagtgtg cccacgtgat tgaacatatt aaagacaatt aagaataatt
     2581 atttttactg attcttttta acaacactga tccaaaggct gaaacattcc ttgtataatg
     2641 ctgaaacatt ctttatataa cacacattcc aacttatgaa ggagtttcaa atactgctct
     2701 aggtgtgagt taaccagtca tttcaacaga atgtttggtg acacaggcta ctatgtgttt
     2761 cataatacca agtcaggtaa cattgaccag ccctctttaa tttttcattc ttaggccatc
     2821 tttataaagg gacttaagaa acaagaatat tagtaagctc agttgtaatc atgttcccag
     2881 aaaatgaaaa tcttttatta tatttgacct ctttctaaca ggggatatag caggctgata
     2941 aattttcttg actgttttat atttcccatt ctcttcttca aagattagtt ttaaactggt
     3001 gggcattacc attgtataaa atgagagttt caaaattgca aaattttaca aaaaacattt
     3061 tcagcttatc tagatctgcc aagcaaaaaa ttgtctaaga catcatagca tataatcaca
     3121 gttttctaat ctaccaccct gtcaggggct aaaaggaata ctagatttcc agtaattaaa
     3181 gaattaaaaa aaaaaaccct accagatatt tgatgattat attcttatag ccaggagatt
     3241 cttagtattt tagaactttt gctacttaat aactggacat actatgttta taaacatatt
     3301 aacctattgt tttgatctgg caaaatgtga catttattaa acgcgtattc agcgaagggc
     3361 gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag tttgtattat
     3421 attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta
     3481 ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa
     3541 atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta
     3601 tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca
     3661 agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca
     3721 taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc
     3781 agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac agaaagagtt
     3841 tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat gcctggcagt
     3901 tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3961 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4021 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4501 ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgcgcgta
     4741 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct
     4801 ctgctttt
//
