LOCUS       sequence-157001-        5328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1932
FEATURES             Location/Qualifiers
     source          1..5328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(187..642)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(324..345)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     536..555
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     840..862
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     854..871
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     855..871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3107..3123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3107..3123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3120..3142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3131..3147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3155..3161,3162..3179,3180..3185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3200..3221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3338..3355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3509..4097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3589..3608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4268..5059,5060..5128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4891..4910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5129..5233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5301..5319
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      241 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc
      301 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      361 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      421 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      481 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      541 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      601 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg
      661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattggag gctacagtca gtggagagga
      901 ctttccagtc agtcagtcag tgaagactgg atgggcgcgc ctgacagccg cgttctacgc
      961 gaggacctgc cccagagttt aaatgtcaat gataagaaaa gagggtgctc aggcaggcgc
     1021 taactttcct taatatccac gccagcgccg tcctcattgg ctgcccggcc cgcgtgacgt
     1081 catggcggct agagttgggc acagctctgc gccgactagt tttccggccg ggcgggagcc
     1141 tgcttctccc cacccagggt ccggccggct ccaacccctg ctttggcctt ccttggcccg
     1201 gcctgtgact gctctagccc ggcccggtcc agcctgggcc gccgtcctgt atggcgtaga
     1261 gtcgatcccc aggaaactag tcttgccgag tgcgctcctc cgcaccttcg ggcactaggg
     1321 ccccaagatc tctgccctta ggctggccag ctacctctct gggtaatcac cctgtcccct
     1381 cctccgatcc ccaagaggcc tcctcagtgc gcaggcgccg ttcgggcccg ccgccccctc
     1441 ccaaggcgtg gggtcccggg gacagacccc gagcccctct ctgcgcaact accgcctcta
     1501 aaaaaaagac catctggtaa ttgtctttct ttagatcttc ccttaattat gcttttaatt
     1561 aaagatggtt caaggcaccg gaccacatga agggcagtaa ttacaaatta cattacaaac
     1621 ccgacagacg tagcaggatc tgctgggaaa aaggtaccgg aataaaaatt tgactaaagt
     1681 ttgggataaa ttaatcggtg gcttgaagaa cagtagcaca tttctgatta tgtaggctcc
     1741 agccaggatt taagttttgt gtcatgattg ttttcatatt tctttgacat ctattgattt
     1801 ggaggcagct gcaagccttt gtcacaggag agaagagcag tcacaatata ttagcttgat
     1861 ggcataactc gaaccggaga agcgagggga gcctgagtga ctggggcgca catgaccccc
     1921 ctcctggggc ctttgggctc aacccttact tttaacttgt tctaacagga ataatggagt
     1981 agtactacta atgcatggtc agaaggggct tttctgtcta gaacgcagga acactctgct
     2041 taccctttat cttctactcc tctccctaca ccaaaaaaac ttcccaatgg ccccctctcc
     2101 atctacctcc cagctttctg ggactttgaa aggggcaagc ccaacttttt atcccaaacc
     2161 tcacttattg tgattaactt ctcttttggc gtttagatct tcaccccttt aactgtactg
     2221 tgacacaccc tgtctacctc gcctcactgc tttaaaaagt acgaacagga gggggtgagc
     2281 gtggtcgaca aagccgacag acggcaaaca atggcttcta ttctccagct ttcctcgaac
     2341 ccaaggagca aaaccgagtc tccctaacca gcaacacagc ttccaaccaa atgtaccaac
     2401 aacccaccaa ctcacaaagg gaacagaggg acgcttctag cttcataagc cctgacccag
     2461 tgcaggggtc cccgaggctg ctcccgtacg aacacctccc tacctttccc aaaggaaaaa
     2521 aaaaaaaagt cttttgggtg agtggagaaa atagaaaagt aatttcttgt tgtaaatatc
     2581 tggaggtaat tgtgttactg gtgtgagttg ttaggggctg taaaaagata caacggcacc
     2641 gtggggagct caattgggaa gagagagtta caataaagag cccattcaag aggtctgccc
     2701 cccaaaaaga ggtaaaataa caaaacagtc acagcatcat ttctctctgt ttttttttaa
     2761 atgcattatt ttttctgttt tacctatggt gattattgat ctgcctgcct gcaagcaaag
     2821 aatttcaaga agcacatttt actctacaag gccattcctg gtctactttt taactacttg
     2881 gtagacagct taaatccata aaaataaacc gaaaatgagg actttttctc agcattgggt
     2941 aaatactggc cacagcagag ggttactagt tacccttatc taggttggct ctccaatgct
     3001 tccttcctgt ctctcagtcc cggcacccac gcgtaggtgt gtcttccagt cagtcagtca
     3061 gtgaaagagg aagggctgga agaggaagga gcttggcgta atcatggtca tagctgtttc
     3121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5281 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
