LOCUS       sequence-157002-        7006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, 
            Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, 
            Molday RS, Wasserman WW, Simpson EM
  TITLE     PAX6 MiniPromoters drive restricted expression from rAAV in the 
            adult mouse retina.
  JOURNAL   Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 
            10.1038/mtm.2016.51. eCollection 2016.
  PUBMED    27556059
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2016-08-10"; volume: "3:16051. doi"; 
            pages: " 10.1038/mtm.2016.51. eCollection 2016"
COMMENT     Sequence Label: pEMS1937
FEATURES             Location/Qualifiers
     source          1..7006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2396..2415)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        2482..2573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2574..2642,2643..3434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2792..2811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4319..4338)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5054..5587
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      5709..6297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6198..6217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6451..6468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6566..6583
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6567..6583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccatcctcca
       61 gcaaaacact tcctcctgcg cctgaaccag agcgggaaat gaggccgagc cacggttccc
      121 ttttcaaacc cactaatcac tccgcaacat gcaaatgcac cgctcgctct cacacaaaat
      181 attgctttat gcaaagcagc gccggggcct cgcgccagcc gattggatgc tccctctccc
      241 cgcctggtgc aattggtccg tttgtttgaa tatgaataca cttgttttcg gctccgccgg
      301 tgggtcccgg ctcctctccc cggcgcaggg cccccgagcc cgggctctgg cgctcgcctg
      361 catctccccg ctccgcgcac gccgagattc ggcgcggccc gccctgctgc cgcgaacttg
      421 agcggtcaag tgaaggtttc tgggttgggg gtcgcgcccc cactccccct aagctggact
      481 cgggactccc agtcctcgga gggtgatcgg gcagcccagt ccaagcaacc gtgcccctgc
      541 ccactgtact gcagcagcaa cacagagtca gacacatgcc aaggaacaag tacacatgga
      601 cctcactaca gccataaccc cagcagtgtc cccagcccac tgcccctctt cataaacact
      661 gcagccagcc tctgaaccca caaagcgcta agaaccggct ctataaatct cccaaagatc
      721 ccctctgagt gtcaccgcaa accatgggtt ctacagatcc cacactgaac cttcaaggtc
      781 tctgcagtgt tgggacagtg atagcaaacc ttacagccct tgagtgctcc cttagacgct
      841 aaaatccaga aatgtttcca tcaccgtcca aactggccaa ggcctggcac aagcatcgag
      901 ttgggttttg tttggttaga atagaagaag gaagaggaga gaacaaccag agcagccagt
      961 atgtggctgg tgaaccccag tccaggtttc cgcacctcca gtgcagacgt gagagtcaga
     1021 gcccgggctc ggcttcagcc acagacaagt ccggccaccg caagtaaggc ttagagccgg
     1081 gagtcggttc gcaccagcca ctgctcgctt tatttccgcc gctgaactcc gccctcccct
     1141 tagccaagga tagatattat caaaaagaaa aaacagaaaa gaaaaatacc acatcatagt
     1201 gttccccatc cctcctttat ctttgtatct cacaacctca tccctgtggt ccaaccttag
     1261 agaataggga gagggagtta gcccctcttt gtagtccccc tccccaaaaa ccacagacac
     1321 acaaagccaa ttgccaattt ccacttcctt aaaaatctcc aacctgcagc cccgacttag
     1381 atagcagccc tcccaggaaa agacgtggct ccctgttcag ctctcctctc ctctcctctc
     1441 ttctccttgg agcctactga taaattgacg ccaggagcct gagcttctta gcaataaata
     1501 agctccaaat atagaagagg ggggaaaata tgatgagcct ctctgacatt tgtctttaaa
     1561 ataaaactag ctgcacgtca agtttgagct taatttctgg aaataggcgg aagtcgctcc
     1621 ggatcatgca tggaaggcta attgaaaaga tcagtcgggg cgcttgtggc agccttgtca
     1681 ctttgtgaca gtgctccgct ccgggaaatt gcatcgtcac gacaaacggg accgtgataa
     1741 aacgaccctt tccgtcctta tttgtagatc actcagacga gattgaactg cacttgtttc
     1801 cccttcgagg ggagccgcgt tttcagggta gccgaaggct tggggctgag ggggggccct
     1861 caccaaggcg cgggtggggg ccggagcctc aactcgatga gaagtgacag gcgtttgggg
     1921 gatctgggct ccggccggga ccagcgcaag cagggacttt gcggggacac cgcttctcca
     1981 acagagcaag gcctggccca cgtttccggt ttctcctaac ttccttttat tgccttcctt
     2041 tgcttcgcaa gttccatcta cccctccagc tacagagccc cacctctagg cacaggaagc
     2101 ttcccggaaa aagaaaggct gtcccagaaa gagaccgaga gagactttcc aaacttcggg
     2161 catagccacg gcaattccca gtctgacgcg tccccgagtc gaataagggc gacacccctc
     2221 aattagcccg ggcgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     2281 gcagttccct actctcgcat ggggagtccc cacactacca tcggcgctac ggcgtttcac
     2341 ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaacaagg
     2401 ggtgttatga gccatattca ggtataaatg ggctcgcgat aatgttcaga attggttaat
     2461 tggttgtaac actgacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2521 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag aatatgagta
     2581 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2641 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2701 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2761 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2821 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2881 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2941 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3001 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3061 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3121 cgatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3181 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3241 ttccggctgg ctggtttatt gctgataaat ccggagccgg tgagcgtggt tctcgcggta
     3301 tcatcgcagc gctggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3421 ttaagcattg gtaaaggagg aaaaaaaaat gagccatatt caacgggaaa cgtcgaggcc
     3481 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3541 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     3601 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3661 ctggctgacg gaatttatgc cacttccgac catcaagcat tttatccgta ctcctgatga
     3721 tgcatggtta ctcaccactg cgatccccgg aaaaacagcg ttccaggtat tagaagaata
     3781 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg tgcacctgtt tgtaattgtc
     3841 cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt
     3901 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     3961 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4021 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4081 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     4141 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4201 aattgcagtt tcatttgatg ctcgatgagt ttttctaaag gaggaaaaaa aaatggagaa
     4261 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     4321 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     4381 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     4441 tgcccgcctg atgaatgctc atccggagtt ccgtatggca atgaaagacg gtgagctggt
     4501 gatatgggat agtgttcacc cttgttacac cgttttccat gagtgcacac gacgatttcc
     4561 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     4621 tccctaaagg gtttattgag aatatgtttt tcgtcagcgc caatccctgg gtgagtttca
     4681 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg
     4741 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     4801 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     4861 agtggcaggg cggggcgtaa acgccgagga ggaaaaaaaa atgcgctcac gcaactggtc
     4921 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     4981 tgactgtttt tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5041 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5101 aacaaagtta ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg gccctgacca
     5161 agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt gagttcggtg acgtagccac
     5221 ctactcccaa catcagccgg actccgatta cctcgggaac ttgctccgta gtaagacatt
     5281 catcgcgctt gctgccttcg accaagaagc ggttgttggc gctctcgcgg cttacgttct
     5341 gcccaagttt gagcagccgc gtagtgagat ctatatctat gatctcgcag tctccggcga
     5401 gcaccggagg cagggcattg ccaccgcgct catcaatctc ctcaagcatg aggccaacgc
     5461 gcttggtgct tatgtgatct acgtgcaagc agattacggt gacgatcccg cagtggctct
     5521 ctatacaaag ttgggcatac gggaagaagt gatgcacttt gatatcgacc caagtaccgc
     5581 cacctaagca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     5641 ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5821 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttagcc
     5881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6361 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6421 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaggc
     6481 gagagtaggg aactgccagg catcaaacta agcagaaggc ccctgacgga tggccttttt
     6541 gcgtttctac aaactctttc tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc
     6601 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
     6661 gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt aagggcgcct
     6721 gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa agcaacgcac
     6781 tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac tattcatcta
     6841 ttatttatga ttttttgtat atacaatatt tctagtttgt taaagagaat taagaaaata
     6901 aatctcgaaa ataataaagg gaaaatcagt ttttgatatc aaaattatac atgtcaacga
     6961 taatacaaaa tataatacaa actataagat gttatcagta tttatt
//
