LOCUS       sequence-157009-        7463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple253 (PITX3 MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1920
FEATURES             Location/Qualifiers
     source          1..7463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(862..881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1492..2025)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     2741..2760
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(join(3645..4436,4437..4505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4268..4287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4506..4597)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4664..4683
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ggtctcctat acgtctctta tacgacatca ccgatgggga acccagacgc tgagtacgta
       61 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc
      121 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta
      181 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata
      241 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
      301 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
      361 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc
      421 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg
      481 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa
      541 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc
      601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg
     1441 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttaggtggc
     1501 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     1561 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     1621 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     1681 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     1741 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     1801 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     1861 ggctacgtca ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     1921 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     1981 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     2041 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
     2101 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     2161 ccagttgcgt gagcgcattt ttttttcctc ctcggcgttt acgccccgcc ctgccactca
     2221 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca
     2281 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2341 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2401 aaactcaccc agggattggc gctgacgaaa aacatattct caataaaccc tttagggaaa
     2461 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2521 aaatcgtcgt gtgcactcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2581 cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca ggcgggcaag
     2641 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2701 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2761 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2821 ctccattttt ttttcctcct ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     2881 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2941 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     3001 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     3061 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     3121 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     3181 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga
     3241 caattacaaa caggtgcaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     3301 ttcttctaat acctggaacg ctgtttttcc ggggatcgca gtggtgagta accatgcatc
     3361 atcaggagta cggataaaat gcttgatggt cggaagtggc ataaattccg tcagccagtt
     3421 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     3481 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac
     3541 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     3601 cctcgacgtt tcccgttgaa tatggctcat ttttttttcc tcctttacca atgcttaatc
     3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata
     3781 ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg
     3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct
     3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4501 ctcatattct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat
     4621 taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc
     4681 ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     4741 gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca
     4801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattag
     4861 gggggctgga tcgcttcgtg ttccccatcg gtgatgtcgt ataggaagca gtatacgaga
     4921 cctataggag acgtatatgg tcttcttttg gcgcgcccgg ctgcggcggc gatctagagg
     4981 gcaggcaggg gccaggggcc gggcacccgg ccggagtggg ggccgccccc ctgctcccgg
     5041 gccgcctctc cgctcgggcg ctcctggact ctcggaggga gtgagcctca ccgcgtactg
     5101 ccacccccag ccggcgccca ttcactttat ggcagaccag ggcgccccca gcccgccgcg
     5161 gcgagccgcg cgcgtcaggc cccgcccctt tccagctgtc ctgctggggc tccgcccttt
     5221 ccagctgtgg atctccaggc cccgcctttg agggaggggt ctggccggcg agacgccaag
     5281 aaccccgccc tctggccaat cagaagcgct cttcagcaac tcggccgcgc ccctccccac
     5341 gtggcagaga ccgcgctccg gctaggacgc ttaggcagag ccaagtgggc gagagtagag
     5401 tggtcccggt agccacgggt aaaagggatc ggctggcagc gaagtgggtt gggccgctac
     5461 cccgaggaaa gtggatcggc gccaggtggg aaggcgcacg gggccgccgg gtctcagcgc
     5521 tcagacttct ctgccactca agtttcgccg attttctcca cttgtcgctg tccgtctctc
     5581 ctcccttttg tctctgttcc aatcctccca cgtctcgcct ttctttcctc ttctttctcc
     5641 tcagccgctt cgctcctgct gtttaccatt ctccttcctc tgccacttta aaagactcat
     5701 ttctctttct ctctgctcct ctttcctcat atctttcctc ttcttcattt cccccttctt
     5761 ctccaaggaa ggtgacttcg tggagacagc agggtttgga atgatacaaa ccaaagtagt
     5821 attccaacgc tgccacgaac tgcaccatca ctgagtgaac ttggacgagt aactatcatc
     5881 tccctccccg cgcaaaacaa ttaagaggag gttcctgctg gatctcaggg cttgggctgg
     5941 gcactccaaa ggcattgcct ttcagtctgt acccttgtct tgcttaggct gtgctctccc
     6001 tccttcagca gggctggttt ctcgctggtg gtaccaggtt aagtcctgtg ggcaaggtgt
     6061 ggccttggtg cctgctggtt aggaatttgc aagggacagg acagatgacc aaggaacatc
     6121 ctggcagact gtgacttcgg ccctctctga cttaggcctc aatttccagg aaacaggctc
     6181 cttctcttct cccagttagg ctatgttgct tgggtgctcc gaagagccta agcccaggcc
     6241 agacactgtg gctcatgcct gtaatcccag cactttggga ggccgaggcg ggcggatcac
     6301 aaggtcagga gattgagacc accctggcta acacggtgaa accccatctc tactaaaaaa
     6361 tacaaaaaat tagccgggct tggtggcggg tgcctgtagt ctcagctact ggggaggctg
     6421 aggaagaaga atggcgtgaa cccaggaggc agagcttgca gtgagctgag atcgcgccac
     6481 tgcactccag actgggcgac agagcaagac tctgtctcaa aaaaaaaaaa aagagcctaa
     6541 gcccagtcac acaatatgcc cctaaggcaa atctgagcag tcatgtcttg actgttcagt
     6601 gactcccatt gcccttagga taaaggtcaa gctccctagc ctggcataca agaccttcag
     6661 gacctgacct tgtcttcatc tccaatttcc tagtagtgtc ctgcacacct ctggcccttc
     6721 ctgtgaaagt aacccatctt tatattgtca attcctactc ctccaatgtt ccactcaagt
     6781 cttattttct ctgggaagcc ttttctgaac tctcagttta gccccttgct ctcatgaccc
     6841 tcagtgtata ccttctatta caccacccac catattgaag ggatcagctt atgtgttgtc
     6901 tgctatgata ataaaattta ggagggtaag gaccatgtct gttattccca ggcataatgt
     6961 agaatctcaa taaatgtcac actgaaatct atgaggaaga tgttctgcct ggtaagatga
     7021 tgattagtgg ttaggaggga ctagggaatt gtcatctctc tcctccccat ctctattgca
     7081 caatcacatg cttttagaat gatgaatttc tggaggtatg tagaagtaga tattggcgag
     7141 cagaaatttt tggcccactt agaggatttt ccaatcggct ttccaggacc tctcaatctg
     7201 agacaggccc aggacaccaa ctctcagttt tcaaatgaag aggaaaatgg ctttgaggta
     7261 atgtggaaag aggtgctatg atggtatatc ctctttccct gactctcttt gggtgataga
     7321 ggctagatcc tcagctggac ccaccgacca gaagcccaat gtgcaggtgt aggaagttac
     7381 tgaggggttc ttcacgtctg atatctgatg ggtgctttac actagagcat cgtggccagg
     7441 tggccggccc cgaagacact ata
//
