LOCUS       sequence-157010-        7835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Baumann BH, Shu W, Song Y, Simpson EM, Lakhal-Littleton S, Dunaief 
            JL
  TITLE     Ferroportin-mediated iron export from vascular endothelial cells in 
            retina and brain.
  JOURNAL   Exp Eye Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728.
  PUBMED    31323276
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Eye 
            Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728."
COMMENT     Sequence Label: pEMS1938
FEATURES             Location/Qualifiers
     source          1..7835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3225..3244)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3311..3402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3403..3471,3472..4263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3621..3640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5148..5167)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5883..6416
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7027..7046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7280..7297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7395..7412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7396..7412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccacccgaag
       61 gcccgcagaa cccccaaggc cgtgctgcgc ggcgccctgg gcgggccctg gtgcctttgc
      121 gcccgcgctc ccggctcttg gccccagtcc gtttgccccg cgggtctgtc gcacctcctg
      181 ggtctgccag ctcctgccgg ggggtaccct ctttgaaggt tcgggggcgg attctgtccc
      241 ccgggcccgg ccccccggcc cgaagcagcc aatccgtgcg cgggtcatcg tgccgcagcc
      301 aatcacagag cctctggcag ctgaagttag ggaaacaacg gctcttgagg ggtagctgag
      361 ggcgcgggac cgctccccgc ccggcagccg cccccagccc cacccgccgt tgtcctagtc
      421 gcggccgagc gcattctggg ctggcctagg gcgcgcttct tgggccgcct ccctgcgcgt
      481 cccggccccg tcacttcaga aggcgctcga cccccagtct gcaccaccca gtccactccc
      541 acatccccga ctgagccggg tggtctcttc cactcgagcc agctccactt ccctgaccaa
      601 ggtttgcaga agccccccac cccgtcaccg ccgtagaggc cctcgtaggg gggctctcca
      661 gaaagcccgc cccggggtcc aagccccacc caggcccttt ctcgcactct cactctccag
      721 ccccgctctg agtcccagca ctgtctctct catcccatgg caaacagaga ggccaggcag
      781 gcgtgtggag gtcaggccca gggccccagc ctcacccccc atgccactca ctgcctctct
      841 ggagcctgag tctctggcaa aaagcggtgg cacaggggct ttccccctgc ctggcgaccc
      901 ccactctccc aagccgcagg ggcttcccag acctctcaat cttcacaggg gctgctcctc
      961 ttcctgcggt actgcccgca ccccatcctt gggggtccag ttcaggtgac accacttcag
     1021 gaagttccca gtgacccagc acactgggtg tagcacccag gggcagtggt ggccccaggc
     1081 ctagcagcct gctctggcct tcagacagga gagacaaagg gacacggagg ggctgtgccc
     1141 tgccctccca ccagtggcga tggtgtccct ggcaccccag cccccaggcc accctccgga
     1201 agccaacttg gagtttcctg gccaggagga agagccccca tccttgctgg aggagggagg
     1261 cgcgggattt ccgggcattc ttctgcacaa tggcccagcc atttctcgaa aggtccactg
     1321 gtgtggacac tccgggcccc ctgaggctca ggtgggggca cgcggctcca aaacccacag
     1381 ccccatgggg gcaccagccc ctttctagta gttctttccc cagtctctct cggccccttt
     1441 gtttccagta aagtttgaac tccaggccct ggactgaggc cactgggtgc ccagttcttt
     1501 tatctggtct gtgctcagtt ccattggaca gggccctcca tagaaaaagt atggcactga
     1561 ggctggtcac agatgtggga ggtttgatga ctttctccct cctggggccc ggttcccttc
     1621 tcccccttca gatggctctg agatggtaaa tgagataagg ccagcgtctg gctcacctag
     1681 tcagggaatg gagttggagt aagacagggc aattttaaat tttctttata gtttgagctt
     1741 tctttctcaa atacagctgt tatctttata ataaaacaaa cacaaaaatg acaagaaaat
     1801 cagtaaaagg ggactattgg ccaacaattc tgtcttccct tcccagtgcc tctgccagtg
     1861 tactcatcca tccaccagca gctatggccc aggggcgtgc agcctggctc ctcagaatgg
     1921 cacatggagc tgggctcctc ccaggagcca cattccttcc cacctccccc cagaggcagg
     1981 catgggcaac tgaatgcaaa caagatgaag aggaaggaca ctgtaaacta aacaattgtt
     2041 agctgccaga cgcagccaca acctggagtc acagagccct cctggactca gggccaaaag
     2101 agacctacac aaggtcacat agtgacccag tccttcccag aaccagtagt ctttggagct
     2161 taaaaacata agcataggta atcttaccat ttaaatctag ttttcctgaa gatgcctgtg
     2221 tgcctttggc caacaggatc tttgtccaag gtgacaatga agatagtata ccatgagccc
     2281 atactgcctg cagagtcgtg gctgccaaaa ttaacagctg tggaattatt tgtgaccctg
     2341 ttaaggataa acatatttgt cccttttcat tttatgatca gcaactgcta aagttcttac
     2401 ttgtaattgc aatttactgc cctcactgta ttgcttagtt tagaggtgga cacagaaaga
     2461 atggaacatt tgttttcttt tgggtctgag ctatgaattt gggccgcatt atcatgcagg
     2521 ccttggcccc tctgtctctg caagattggg ctttcctgtt tttgtcattc tcagcttact
     2581 tcgcaggcat gctttgtgta gaggagaaaa ataccctcca aagaacctcc caaggaggtt
     2641 accaggtaca cggttcagcc aagtgggtga ctcaatggga tgcatctctt tgtgtttgtc
     2701 agggccggga agggtgtgac agcatgtggg gagagactca gttcccagcc aaataacttt
     2761 accacctgag ctatgaggca gttcctgcac atcaccactc cagcagggtc cagccttgct
     2821 gcggcagaaa gcgagtgtgt gggaaagcaa atggctccac acgacgcttg cagtgttcag
     2881 agggatgagt atgaattgtg tgtatctcag ggaccccttt gacccatgaa tacatgtata
     2941 gagtattctc tattatgtgg gtgaagtgta tctacttaca tacttaagta tttgtgagta
     3001 aaacgagaaa aaaaacgcgt ccccgagtcg aataagggcg acacccctca attagcccgg
     3061 gcgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     3121 ctctcgcatg gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     3181 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag
     3241 ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca
     3301 ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3361 taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagtat tcaacatttc
     3421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3781 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3841 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3901 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc gatggcaaca
     3961 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4021 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4081 tggtttattg ctgataaatc cggagccggt gagcgtggtt ctcgcggtat catcgcagcg
     4141 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4201 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4261 taaaggagga aaaaaaaatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     4321 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     4381 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     4441 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     4501 aatttatgcc acttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     4561 tcaccactgc gatccccgga aaaacagcgt tccaggtatt agaagaatat cctgattcag
     4621 gtgaaaatat tgttgatgcg ctggcagtgt gcacctgttt gtaattgtcc ttttaacagc
     4681 gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     4741 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     4801 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     4861 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     4921 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     4981 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     5041 catttgatgc tcgatgagtt tttctaaagg aggaaaaaaa aatggagaaa aaaatcactg
     5101 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     5161 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     5221 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     5281 tgaatgctca tccggagttc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     5341 gtgttcaccc ttgttacacc gttttccatg agtgcacacg acgatttccg gcagtttcta
     5401 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     5461 tttattgaga atatgttttt cgtcagcgcc aatccctggg tgagtttcac cagttttgat
     5521 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcactatggg caaatattat
     5581 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     5641 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     5701 ggggcgtaaa cgccgaggag gaaaaaaaaa tgcgctcacg caactggtcc agaaccttga
     5761 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     5821 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     5881 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
     5941 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca
     6001 tgcgggctgc tcttgatctt ttcggtcgtg agttcggtga cgtagccacc tactcccaac
     6061 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
     6121 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
     6181 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc
     6241 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
     6301 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
     6361 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaagcag
     6421 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     6481 tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6661 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga
     7321 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
     7381 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
     7441 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
     7501 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
     7561 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
     7621 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
     7681 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
     7741 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
     7801 ataatacaaa ctataagatg ttatcagtat ttatt
//
