LOCUS       sequence-157011-        7898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1941
FEATURES             Location/Qualifiers
     source          1..7898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3288..3307)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3374..3465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3466..3534,3535..4326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3684..3703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5211..5230)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5946..6479
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6601..7189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7090..7109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7343..7360
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7458..7475
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7459..7475
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccagcggttg
       61 gtgggcgctg cccgagtccc ggccggcagc gcccgcctcg ccgccgggcg ccgccgctct
      121 ccagctgccc tccagcctgc aaggctcctg ggcgggacag gtgggtgtca gtcgcgcgct
      181 gcgctgctcc ctgggcgctc agaattcgtc caacagtgga aatttaagca tccttaagtt
      241 tcttcccggc tcttgcaaaa ccgcagctgc tgggaatgct gcttgatatg gacacacaca
      301 tggacagaca gatggagaga tggacaggaa gactgaggaa gaaggaagaa cagcaagaga
      361 gggatgttga aagacagaga tggagacacg aaaatggcct tcctacggag cgaaatccct
      421 ctttttcccc attcctgtca ctcagtcttc gatctctccc tctacatagg catgcagagt
      481 gtgggcaatc tctgccgacc gctggtctcc caaggaaaga aggaaaacac taccgcagtc
      541 tcagaggcag acgggctccg cgctgccgga acccaagcgc agagtgggaa aactagattg
      601 ctgggaaatc ttccgaggga agaggcggcc aggtccgggt ttccggggag agccgcgcac
      661 gcagcggcga agggtccccg aagttcatta ccccgtgact ttgtgcctgt cgctgcgggc
      721 tgggtcccct gtaatatctc cagaagcgct ctgacaggca gccgcgggga ggggaatagg
      781 gcgtccctgg cccacttagc ttttccccca actcctggcg gggtctgacg tcagccatgt
      841 gcggggaggg gatgggaaga gggagggggt taaatgctaa tgatattaat gaacctccaa
      901 gcggctccca aagaaaatgc aggcgctgtc ggagcttacg agttagaaag tgtgaatatg
      961 ggagtgtggc tctgtgcgtg cgtgatggtg aggacggtgc ggctcgtaat aaaaatgaat
     1021 tcgtatattt cacggcaaga aggaagggga ggggtgacgg ggctttctca aaacgctttt
     1081 ccccctctct ccagagtgcc agatagattc gcggaagaaa ttgggtggct gtgtgctggc
     1141 gtgtgattgt gcgcgccgga catggccatg tgagttggtt cattcttggt ccggtgcccc
     1201 ttctgttggg taaaacttgg atctggcccg aggtgagtaa tttactcatt ttgcacatat
     1261 ctacggaaag tcagggctga ggatggggtc ttctgtagaa acaattgtta tgtaaaacca
     1321 ggtactccag tttgaatgtg gccaatatat ttcagaataa gctacaattc tttattatgt
     1381 ggatgagtat atcaggacca catatttgat ctctgccaat gaagtgaaaa gcatcataca
     1441 ttctactatg ctttgtttcc aacagatcta gttcacacag tactctgttg tcctattcta
     1501 gtaagtaatt tcagaattca catcacttac aaagatgccg agtggctatt tagggaactc
     1561 tgattcaaaa aaaaaaaaaa aaaaccctcc aaaacaaaac aaacctccct ctctcttctc
     1621 cctcccccaa accccacgtg ctttgtgcaa gacggagtca accagtggga ttcacaggag
     1681 gtccccaagt caaatgctgc agctgcgttt taaaaaaggg ttggataaac tacagccgtt
     1741 aataacatct gtatctatgc aagctaagat gatgataggg gtaatcagat ctcatgcttc
     1801 agggcataag atgattgcct gctgggagga ggaggatttg gcctttcagg tacaggagtt
     1861 cacaactgag gaggtaggtt gcagacattt ctccttagcc tctaaaattt caggtgtaac
     1921 ttgctctctc agcataaagg ctgatagctg ttgcagttgc aattttctct ctaaacccaa
     1981 ctccttttgt tgacttgcag aggcttacat tccaaaaaag gtaatttgaa agtgctgctg
     2041 aatttagatg cattttgcta atttgttttt attatgcata gaaatattgg tataaaaggt
     2101 gtttcaatta ttttctaagg gagggaccta tgtattctct gcagccacaa agcttttaaa
     2161 tggagttatt tttctcggcc tatagccgct ctccctttta agtaccccag cagaaaaagt
     2221 aagtttactg ctcctcgcac aatgggcctc ttcacttttc aggtttggga gacctgggtg
     2281 acctacgaaa gttaattaga aggttaacaa agacgaagac agagtcgaat ccatcgtttc
     2341 tccttcagcc aaagatgtca tccattaatt agggaaatcg ttaaggtggc ctttatagaa
     2401 tccatagaaa atctgtcgtt ggcggtcgac agttttgatc agattttcct ctccccaata
     2461 atgctatcct taatggtacg aaaagcagag ggccggggca gggagagggg cgcggggagg
     2521 gggtgggaag gatgtgtttc agcaacagta cagttaacca gccgccggtg ctgacggctt
     2581 tgattctcag gctgccccgc atacttgctc catgatgtaa tccttattga acctggaggc
     2641 cgagggagcg ggaggagcct atagtttatg gctggaacca ttaagggtgc ttgataactg
     2701 ctcattaaat aaattaagtg cctaaaatat aattactgat gttctacttt tcccctgtct
     2761 ttgctgcacc acaggaaaaa gtttccacgg ccattgattt atatgagcaa aaaccaaaat
     2821 taaattaaga ggtcacacac cagtagcaaa aacaaaatga ggaaaacatt ttctaatgtt
     2881 ttactgcaaa ggaatcgggg cttttacgta atagcaaaat tagggtttag atggtagtat
     2941 tctttcaacc aacttttcac aaatatttcc tggaactggc ccaaggaatg aatcctaatt
     3001 tagcctaatc tttttcagat gtaattgaat tcacttgttt aggtttctgc ctcctccacc
     3061 tttcatccgg tggcaacacg cgtccccgag tcgaataagg gcgacacccc tcaattagcc
     3121 cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3181 ctactctcgc atggggagtc cccacactac catcggcgct acggcgtttc acttctgagt
     3241 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa ggggtgttat
     3301 gagccatatt caggtataaa tgggctcgcg ataatgttca gaattggtta attggttgta
     3361 acactgaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3421 caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag tattcaacat
     3481 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3541 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3601 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3661 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3721 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3781 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3841 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3901 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3961 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcgatggca
     4021 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4081 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4141 ggctggttta ttgctgataa atccggagcc ggtgagcgtg gttctcgcgg tatcatcgca
     4201 gcgctggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4261 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4321 tggtaaagga ggaaaaaaaa atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     4381 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     4441 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     4501 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     4561 cggaatttat gccacttccg accatcaagc attttatccg tactcctgat gatgcatggt
     4621 tactcaccac tgcgatcccc ggaaaaacag cgttccaggt attagaagaa tatcctgatt
     4681 caggtgaaaa tattgttgat gcgctggcag tgtgcacctg tttgtaattg tccttttaac
     4741 agcgatcgcg tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     4801 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     4861 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     4921 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     4981 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     5041 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     5101 tttcatttga tgctcgatga gtttttctaa aggaggaaaa aaaaatggag aaaaaaatca
     5161 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     5221 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     5281 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     5341 tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     5401 atagtgttca cccttgttac accgttttcc atgagtgcac acgacgattt ccggcagttt
     5461 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     5521 gggtttattg agaatatgtt tttcgtcagc gccaatccct gggtgagttt caccagtttt
     5581 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcactat gggcaaatat
     5641 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     5701 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     5761 ggcggggcgt aaacgccgag gaggaaaaaa aaatgcgctc acgcaactgg tccagaacct
     5821 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     5881 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     5941 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     6001 taggtggctc aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat
     6061 ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg tgacgtagcc acctactccc
     6121 aacatcagcc ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc
     6181 ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt
     6241 ttgagcagcc gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga
     6301 ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg
     6361 cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa
     6421 agttgggcat acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaag
     6481 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     6541 agttacgcgc gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6721 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag cccaccactt
     6781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag gcgagagtag
     7381 ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct
     7441 acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt
     7501 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
     7561 ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt
     7621 gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata
     7681 agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat
     7741 gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga
     7801 aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa
     7861 aatataatac aaactataag atgttatcag tatttatt
//
