LOCUS       sequence-157013-        7886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy plasmid containing Ple267 (UGT8 MiniPromoter) insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1944
FEATURES             Location/Qualifiers
     source          1..7886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3276..3295)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3362..3453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3454..3522,3523..4314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3672..3691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5199..5218)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5934..6467
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6589..7177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7078..7097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7331..7348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7446..7463
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7447..7463
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccgagtttga
       61 gtgtgtgtct gccgcgtaat tgtatccggg acagaaggga cctgtgtttg tgcgctgaat
      121 caggtgtcgc cgccttcggt ggttcggggg cgtaagggag gcttcggagt tagcgtgttg
      181 gctaaggagg gctctctggg ttcacagttg atagcaatgc tttaaccgtg tcctccctcg
      241 ggtcctcagc gcgcctgctc gtccgcagct cccagcgggc ggccagcccc ccccgccctg
      301 cgcgcggcga gcagagctgg agcgcgccgc tgcccttccg cacttggctc agcggggcac
      361 gacacgggcc gccacgcgct cagcctagca tcactcgcct ctgactgcga gcgcctccag
      421 ctccccgggc tggggcatgg atgcagacgc gccttcgtac cacgcgctcg caggctgggc
      481 aaggggcagc gcgttgggat ccggccagtc gtccgctgag cgcggttggc ttgcaggctg
      541 ggacttttcg cgagccctct gggcgctcga gttgcgaact ctgacaccgg cacgtggccc
      601 cgcccactct tccctctttg cgaggcgtgt cccgcgccct ttctgccacc acccacgtag
      661 tccctgtgct gtagcggaaa gccttaaaaa acgcgagttt ggggatcctg aaaaaaagta
      721 aatagggttg ctgggctgaa taacatccct cctctcaggc tcctgctgaa agggccattc
      781 atgatttgcc tggttgagcc tgtggcgact ttattctgac gaatctgcct ttgccagcag
      841 cataaccttg ccaagttggc gtcttgacca gcaagcaact gtgcccaagg aagcctctct
      901 gtggtgcctc agacctgaga ggccgagtgc ctttgagcgc ctgaggtaga ttgtgagttt
      961 catgaaaacc gagaaagtct aagtgtgcat gtgcgtaaga tattttatat atatatatct
     1021 catatatata tgagatatgt ctatataacg tggactgaat tgtttattgt ttatgtttcg
     1081 tgtcgagcat aaattccaga agctaagatg cttccatagc acacaaacgt tatgcgttta
     1141 aacgttggaa atcaaaaatg ccatgtcagg tcattctgtc cttgttccaa agtgagattc
     1201 agaaataggt tgggattttc tgcccttaga taagaaaacg gaaattatat tttactgtat
     1261 atgttatcag ttaatgataa attaaaaaac taatatacac tgctgttatt gtaaaatcca
     1321 tatgctcaag aatgttcatg gatggagaga tcttcaaatg ccatttcaaa aaagaaaatg
     1381 cacttttctt ctttgcagat taggaatttg aacatattcc ctgtgcaaaa tgcagggaat
     1441 attttcacag cctttgtacc tgattaccat actctctatt tatttcttat ttaaattgca
     1501 ctagaaaacc taaagtaaat gaaatgtaac ataataaaga aaagagaatt tagacattta
     1561 ctttaaaagc tttatttctc aaaggtcaag cttcagtttc aagctactgt ttgatgatgc
     1621 atatgtaaat tcttggtaaa aacttgatca ttagcaatta aaacaacttt tttcttttat
     1681 tatataaaaa tgtttaaaat tgtttcacca catccttgtc cttaaaatgg tgtaaactga
     1741 ccattgtgta aggtttttgt cagatttttg gatagaaaag gagtatatat tatgtgtaga
     1801 tatgtccatg tttattgttt ctctaatcta tctatttttg tccacattaa atactgatta
     1861 ttgaatttct cacagctcta cacagtaagg gtgttccata atgtaaaaca agtttattca
     1921 atcatcatat gaatagttga aatacacttt tggagtttga aatgataaaa gcaagaaatc
     1981 agtgttgtga acacttgaag aggcttcatg gagacaggtg gtacaattta tacaggttat
     2041 tttataagtg ggctaatagt tgatgtctat ttttcacaaa agaaccagat gaagtttata
     2101 acttaatgac ctcatttctg aatccaggtg tgtcataaat agagcatatg atgcaaatta
     2161 tgttacctat tgagaacaga gtaaacaaat ttatacaaag caggctgttt ccattatctt
     2221 aacatcactt cttgatttac taagcctata caggcaccaa gctgctttat aacctgcaat
     2281 cacctggaac cccgtggaac tctgcagttg atgaccctcc ctcttttggc ctttcctttc
     2341 ccccagtgtg ctgcacttag gagcagaaga tacatttata aaatttcgtt aaaattatta
     2401 ccacagttat ttcacaattg ttactaaaat acttcttagt gcaagtggaa caaaaggaag
     2461 gaaggaccaa agccttcaaa atgttcaatg ataagcttaa agctgccatt ctttctgtgc
     2521 attgtgggga aaaggaggag gagtttgtgc tgagttcctg tgatgactaa tgaaattttt
     2581 attcttttca ataagagctt taacaattag gctgtgttat aatgaaatgc ccatttgtcc
     2641 aaagtctgat tcctggtcca taaatacctc tttgtcagag gtacatccct aaaatatgcc
     2701 tttgtcttga gtggaatgca accagaggcg tttagtgcaa ggagtctcag taaagcagcc
     2761 tgaaataaac aatggggctt cagaaaagct gaaatgaaaa tgtatatgta tatgtttttc
     2821 tgtaccatgt gctattcatt acctgactaa aagattatct acaacaatag ggagtaaaat
     2881 ttcacactta aatatgatca tagaggaatg tttaaagaat ccaaattaat gaaagacatt
     2941 ttacattctc agtaacagtg taaaggacat ctggaaacta ccttttttgt tgtatatttt
     3001 taaaggattt tagttcaaaa ctaactaatg attttaaata gtttgtactt tcctagaaat
     3061 actttacgcg tccccgagtc gaataagggc gacacccctc aattagcccg ggcgaaaggc
     3121 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     3181 ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     3241 caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga gccatattca
     3301 ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac actgacccct
     3361 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3421 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc
     3481 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3541 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3601 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3661 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3721 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3781 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3841 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3901 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3961 gccataccaa acgacgagcg tgacaccacg atgcctgtag cgatggcaac aacgttgcgc
     4021 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4081 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4141 gctgataaat ccggagccgg tgagcgtggt tctcgcggta tcatcgcagc gctggggcca
     4201 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4261 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaaggagg
     4321 aaaaaaaaat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     4381 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     4441 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     4501 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     4561 cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     4621 cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata
     4681 ttgttgatgc gctggcagtg tgcacctgtt tgtaattgtc cttttaacag cgatcgcgta
     4741 tttcgcctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     4801 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     4861 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     4921 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     4981 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     5041 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     5101 ctcgatgagt ttttctaaag gaggaaaaaa aaatggagaa aaaaatcact ggatatacca
     5161 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     5221 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     5281 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     5341 atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     5401 cttgttacac cgttttccat gagtgcacac gacgatttcc ggcagtttct acacatatat
     5461 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     5521 aatatgtttt tcgtcagcgc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     5581 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc
     5641 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
     5701 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     5761 acgccgagga ggaaaaaaaa atgcgctcac gcaactggtc cagaaccttg accgaacgca
     5821 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt
     5881 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     5941 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa
     6001 gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg
     6061 ctcttgatct tttcggtcgt gagttcggtg acgtagccac ctactcccaa catcagccgg
     6121 actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg
     6181 accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt gagcagccgc
     6241 gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg
     6301 ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct
     6361 acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac
     6421 gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaagca gagcattacg
     6481 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgcgc
     6541 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6721 gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca agaactctgt
     6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7321 gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg aactgccagg
     7381 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc
     7441 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag
     7501 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
     7561 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg
     7621 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct
     7681 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
     7741 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
     7801 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
     7861 actataagat gttatcagta tttatt
//
