LOCUS       sequence-157027-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of LOV V416L, a mutant with super long 
            relaxation time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-LOV2 V416L
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     303..322
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     364..383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        364..382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    383..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        423..532
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(692..710)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatataagc agacgtcgtt tagtgaaccg tcagatcact agatgcttta ttgcggtagt
       61 ttatcacagt taaattgcta acgccagtct cgaacttaac gtgcagaagt tggtcgtgag
      121 gcactgggca ggtaagtatc gggccctttg tgcgggggga gcggctcggg gctgtccgcg
      181 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
      241 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      301 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgg atcggaccga
      361 aattaatacg actcactata ggggaattgt gagcggataa caattccccg gagttaatcc
      421 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
      481 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
      541 tataccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      601 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      661 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      721 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      781 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      841 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      901 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      961 cctgtaatgc agaagaagac tatgggctgg
//
