LOCUS       sequence-157031-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of LOV I427T, a mutant with super short 
            relaxation time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-LOV2 I427T
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(200..223)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(230..262)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(302..730)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPTIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat
       61 gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga tcgatctcag
      121 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
      181 acctgaggtt aatcacttag tgatggtgat ggtgatggtg gtgctcgaga tcctcggggt
      241 cttccggggc gagttctggc tggcttgcac gtgtatacag ctgtgcggcc gcaagctttt
      301 aaagttcttt tgccgcctca tcaatatttt ctgcagtttt cttaatcagc atgactccct
      361 ctctctcggc agcatctcgg acatgctcag ttccatccaa ctgaacccca ataaagtact
      421 ggacatctcc cttctgatct cgcataggct gcaagtgaaa gaggttccag aactttttac
      481 cactctttgt ataattaatc agctgaacag tgacctctgt ttggttatct atggcatctc
      541 taatttttct cactgtcgcg cgatcagttt caggaccttg tagaaacctg cagtttcttc
      601 ccaaaatttc ttcacggcta tattctgtca actgcaagaa actatcggac gcgaatatag
      661 tgggattatc tggcaatctt gggtcagtaa tgacaaagtt cttctcaata cgttcaagtg
      721 tagtagccaa ggatccagaa cccttgtaca gctcgtccat gccgccggtg gagtggcggc
      781 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      841 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
      901 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
      961 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatagtct
     1021 tcttctgcat tacagggccg tcggagggga agttggtgcc gcgcagcttc accttgta
//
