LOCUS       sequence-157035-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, 
            Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn 
            KM, Dokholyan NV
  TITLE     Rational design of a ligand-controlled protein conformational 
            switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 
            10.1073/pnas.1218319110. Epub 2013 Apr 8.
  PUBMED    23569285
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1218319110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: "6800-4"
COMMENT     Sequence Label: pUSE-Src-KD-UniRapR-mCerulean-myc
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(315..344)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(357..608)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(399..420)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     748..767
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatcat
       61 ggcaggcatg ggacagtcac tgtaccagcc tccacctgtc cttccaggct gccccatccc
      121 tgaagccctt ccatgctcca aaaggggcat tgccgaggca acgcagcccc agagagagga
      181 tgaagggagg caggtgcatc ggaggcaaca cccggaagcc tagaggtgac agttctgcgc
      241 acacagtccc tataggttct ccccgggctg gtactgtggc tctagaggtg acagttctgc
      301 gcacacagtc ctcacaggtc ctcctcggag atcagcttct gctccccgct tccggacttg
      361 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      421 tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg
      481 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      541 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      601 tgcttgtcgg cggtgatata gacgttgtcg ctgatggcgt tgtactccag cttgtgcccc
      661 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      721 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      781 gtgcgctcct ggacgtagcc ttcgggcatg gcggact
//
