LOCUS       sequence-157041-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the super-dark state of LOV2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-LOVSD
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(200..223)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(230..262)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(302..727)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="ATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRN
                     ARFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQY
                     FIGVQKDGTEHVRDAAEREAVMEIKKTAEEIDEAAKEL"
ORIGIN
        1 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat
       61 gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga tcgatctcag
      121 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
      181 acctgaggtt aatcacttag tgatggtgat ggtgatggtg gtgctcgaga tcctcggggt
      241 cttccggggc gagttctggc tggcttgcac gtgtatacag ctgtgcggcc gcaagctttt
      301 aaagttcttt tgccgcctca tcaatttctt ctgcagtttt cttaatctcc atgactgcct
      361 ctctctcggc agcatctcgg acatgctcag ttccatcctt ctgaacccca ataaagtact
      421 ggacatctcc cttctgatct cgcataggct gcaagtgaaa gaggttccag aactttttac
      481 cactctttgt ataattaatc agctgaacag tgacctctgt ttggttatct atggcatctc
      541 taatttttct cactgtcgcg cgatcagttt caggaccttg tagaaacctg gcgtttcttc
      601 ccaaaatttc ttcacggcta tattctgtca actgcaagaa actatcggac gcgaatataa
      661 tgggattatc tggcaatctt gggtcagtaa tgacaaagtt cttctcaata cgttcaagtg
      721 tagtagcccc gggggatcca gaacccttgt acagctcgtc catgccgccg gtggagtggc
      781 ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      841 ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
      901 tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
      961 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccata
//
