LOCUS       sequence-157042-        9362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a codon-optimized ORF of human SF3B1 (wild-type).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Kesarwani AK, Ramirez O, Gupta AK, Yang X, Murthy T, Minella AC, 
            Pillai MM
  TITLE     Cancer-associated SF3B1 mutants recognize otherwise inaccessible 
            cryptic 3' splice sites within RNA secondary structures.
  JOURNAL   Oncogene. 2016 Aug 15. doi: 10.1038/onc.2016.279.
  PUBMED    27524419
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2016 Aug 15. doi: 10.1038/onc.2016.279."
COMMENT     Sequence Label: pCDNA3.1-FLAG-SF3B1-WT
FEATURES             Location/Qualifiers
     source          1..9362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             941..964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4956..4973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4962..5186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5232..5660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5319..5338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5529..5550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5669..5689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5674..6003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5854..5989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5916..5935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6070..6864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6124..6143)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6734..6753
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7038..7159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7075..7094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7129..7148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7208..7224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7208..7224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7221..7243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7232..7248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7256..7262,7263..7280,7281..7286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7301..7322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7439..7456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7610..8195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7690..7709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8366..9157,9158..9226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8989..9008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9227..9331)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccaccatg gactacaaag atgacgacga
      961 caagtctaga atggctaaga tcgctaaaac ccacgaggac attgaggcac agattcggga
     1021 gattcagggc aagaaggcag cactggacga ggcacagggc gtggggctgg atagcaccgg
     1081 ctactatgac caggaaatct acggcgggtc tgacagtaga ttcgccggct acgtgaccag
     1141 catcgccgct accgagctgg aagacgatga cgatgactat agctcctcta cttcactgct
     1201 ggggcagaag aaacctggat accacgcacc agtggccctg ctgaacgata tcccacagtc
     1261 caccgagcag tatgacccct tcgctgaaca ccggccccct aagattgcag atagagagga
     1321 cgaatacaag aaacatcgga gaacaatgat catttctcca gagcggctgg accccttcgc
     1381 cgacggaggc aaaacacctg atccaaagat gaacgccagg acttacatgg acgtgatgcg
     1441 cgaacagcat ctgaccaagg aggaacggga gatcagacag cagctggccg agaaggcaaa
     1501 agccggagaa ctgaaagtcg tcaatggcgc agccgcttct cagccaccca gtaagaggaa
     1561 aaggcgctgg gatcagaccg ctgaccagac acctggagca actccaaaga aactgagttc
     1621 atgggatcag gcagagaccc caggacacac acctagcctg cgatgggacg aaacccctgg
     1681 acgagcaaag ggctccgaga ctccaggagc taccccaggc agcaagattt gggaccccac
     1741 accttctcat actccagctg gcgcagcaac cccaggaaga ggcgacacac caggacacgc
     1801 aactccagga catggaggag ctacaagctc cgcaaggaaa aatcgctggg atgagacccc
     1861 taagacagaa agggacactc cagggcacgg atcaggctgg gccgaaactc cacgaaccga
     1921 tcgaggaggg gacagcatcg gggagactcc aacccccgga gcttcaaaga gaaaaagcag
     1981 atgggatgag acacccgcat cccagatggg aggctctacc cctgtgctga caccaggaaa
     2041 gactcccatc ggcacccctg ccatgaacat ggctacacct actccaggcc atattatgtc
     2101 tatgacccca gaacagctcc aggcctggcg atgggagcga gaaatcgacg agcgcaatcg
     2161 acctctgagt gatgaggaac tggacgctat gttcccagag ggctataagg tgctgcctcc
     2221 acctgccggc tacgtcccaa tcagaactcc cgccaggaaa ctgaccgcta cacccactcc
     2281 tctgggggga atgaccgggt ttcacatgca gacagaggat cgcactatga agagcgtgaa
     2341 cgaccagcct tccggaaatc tgcctttcct gaagccagat gacatccagt actttgataa
     2401 gctgctggtg gacgtggacg aatccacact gtctccagag gaacagaagg agcgcaaaat
     2461 catgaagctg ctgctgaaga tcaagaacgg cacacctcca atgcgaaagg ctgcactgcg
     2521 gcagatcact gacaaagcac gggagttcgg ggccggaccc ctgtttaatc agattctgcc
     2581 tctgctgatg tccccaaccc tggaggatca ggaacggcac ctgctggtga aagtcatcga
     2641 cagaatcctg tataagctgg atgacctggt gcgcccctac gtccataaga tcctggtggt
     2701 cattgagcct ctgctgatcg atgaagacta ctatgcccgc gtggaggggc gagaaatcat
     2761 ttctaacctg gccaaggccg ctggactggc taccatgatc agtacaatgc ggccagatat
     2821 tgacaacatg gacgagtatg tgagaaatac cacagctagg gcattcgccg tggtcgcttc
     2881 tgcactgggc atccccagtc tgctgccttt tctgaaagcc gtgtgcaaga gtaagaaatc
     2941 atggcaggca cggcacacag gcatcaagat tgtgcagcag atcgcaattc tgatggggtg
     3001 tgccatcctg ccccacctga gaagcctggt ggagatcatt gaacatggcc tggtggatga
     3061 gcagcagaag gtcaggacca tctccgccct ggctattgca gccctggcag aggctgcaac
     3121 accatacggg atcgagagct tcgactccgt gctgaaaccc ctgtggaagg gcatccggca
     3181 gcacagaggc aaaggactgg cagcttttct gaaggccatc ggctatctga ttcccctgat
     3241 ggatgctgag tacgcaaact actatacccg cgaagtgatg ctgatcctga ttcgagagtt
     3301 ccagagccct gatgaggaaa tgaagaaaat cgtcctgaaa gtggtcaagc agtgctgtgg
     3361 caccgacgga gtggaggcca attatatcaa gacagaaatt ctgccccctt tctttaaaca
     3421 cttttggcag cataggatgg ctctggatcg acggaactac cgccagctgg tggacactac
     3481 cgtcgaactg gccaataagg tcggagcagc cgagatcatt agccgcatcg tcgatgacct
     3541 gaaagacgag gccgaacagt accggaagat ggtcatggag accatcgaaa aaattatggg
     3601 aaacctgggc gccgccgaca ttgaccacaa gctggaggaa cagctgatcg atggcattct
     3661 gtatgccttc caggagcaga caactgaaga cagcgtgatg ctgaacggat ttggcaccgt
     3721 ggtcaatgcc ctgggaaaaa gagtgaagcc atacctgccc cagatttgcg gcacagtcct
     3781 gtggcggctg aacaacaaga gtgctaaggt gagacagcag gccgctgatc tgatttcaag
     3841 gactgccgtg gtcatgaaaa cctgtcagga ggaaaagctg atggggcacc tgggagtggt
     3901 cctgtacgaa tatctgggcg aggaataccc tgaggtgctg ggctccatcc tgggggccct
     3961 gaaggctatc gtgaacgtca ttgggatgca caaaatgact ccacccatca aggacctgct
     4021 gccaaggctg acccccattc tgaaaaaccg ccatgagaag gtgcaggaaa attgcatcga
     4081 tctggtcggc cggattgcag acagaggagc agagtacgtg agcgcccggg agtggatgcg
     4141 catctgtttc gagctgctgg aactgctgaa ggctcacaag aaagcaatta gaagggccac
     4201 agtgaatact tttgggtaca tcgcaaaggc cattggacca catgacgtgc tggccaccct
     4261 gctgaacaat ctgaaagtcc aggagaggca gaaccgcgtg tgcaccacag tcgccatcgc
     4321 catcgtggcc gagacctgtt cccccttcac agtcctgcct gctctgatga atgagtatag
     4381 ggtgcccgaa ctgaacgtgc agaatggggt cctgaagtca ctgagcttcc tgtttgagta
     4441 catcggcgaa atggggaaag attacatcta cgcagtgaca cctctgctgg aggacgccct
     4501 gatggatcgc gacctggtgc accgacagac tgcctcagct gtggtccagc atatgagcct
     4561 gggcgtgtat gggttcggat gcgaggactc cctgaaccac ctgctgaatt acgtgtggcc
     4621 caacgtcttt gaaacctctc ctcatgtgat ccaggctgtc atgggagcac tggagggcct
     4681 gagagtggcc attggacctt gcaggatgct ccagtattgt ctccagggcc tgtttcaccc
     4741 agcccgaaaa gtgcgggatg tctactggaa aatctacaac agtatctaca tcggctcaca
     4801 ggacgcactg attgcccatt accccagaat ctacaatgac gacaaaaata cttacatccg
     4861 atacgaactg gactacatcc tgtgagaatt ctgcagatat ccagcacagt ggcggccgct
     4921 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4981 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5041 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5101 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5161 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5221 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5641 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5701 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5821 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5941 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6001 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     6061 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6121 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6181 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6241 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6301 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6361 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6421 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6481 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6541 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6601 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6661 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6721 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6781 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6841 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6901 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6961 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7021 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7141 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7201 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7261 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7321 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7381 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9361 tc
//
