LOCUS       sequence-157080-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP-based BiFC vector for transient expression of CN (1-155) in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBCN155
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(2..460,461..463,464..466))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     141..160
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     400..421
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    486..541
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(561..906)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(598..618)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gggcggtgat atagacgttg tggctgatgt agttgtactc cagcttgtgc cccaggatgt
       61 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      121 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      181 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      241 ggtagcggct gaagcactgc acgccccagg tcagggtggt cacgagggtg ggccagggca
      301 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
      361 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      421 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggat cctctagagt
      481 cgactgtaat tgtaaatagt aattgtaatg ttgtttgttg tttgttgttg ttggtaattg
      541 ttgaaggatc tcaagaagcc tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
      601 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
      661 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gttcctcgtg
      721 ggtgggggtc catctttggg accactgtcg gtagaggcat cttgaacgat agcctttcct
      781 ttatcgcaat gatggcattt gtaggagcca ccttcctttt ctactgtcct ttcgatgaag
      841 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
      901 gtctcaatag ccctctggtc ttctgagggg ggatcctcta
//
