LOCUS       sequence-157094-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DsREDmonomer-based BiFC vector for transient expression of RN 
            (1-168) in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBRN168
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     502..521
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      638..890
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(929..945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(929..945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    949..972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(976..982,983..1000,1001..1006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1021..1042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagaggacag gcttcttgag atccttcaac aattaccaac aacaacaaac aacaaacaac
       61 attacaatta ctatttacaa ttacagtcga ctctagagga tccatggaca acaccgagga
      121 cgtcatcaag gagttcatgc agttcaaggt gcgcatggag ggctccgtga acggccacta
      181 cttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct
      241 gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
      301 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactaca tgaagctgtc
      361 cttccccgag ggcttcacct gggagcgctc catgaacttc gaggacggcg gcgtggtgga
      421 ggtgcagcag gactcctccc tgcaggacgg caccttcatc tacaaggtga agttcaaggg
      481 cgtgaacttc cccgccgacg gccccgtaat gcagaagaag actgccggct gggagccctc
      541 caccgagaag ctgtaccccc aggacggcgt gctgaagggc gagatctccc acgccctgaa
      601 gctgaagctg tacaagtaaa gcggccgccc ggctgcagat cgttcaaaca tttggcaata
      661 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
      721 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
      781 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
      841 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag
      901 ctgtcaaaca tgagaattcg taatcatggt catagctgtt tcctgtgtga attgttatcc
      961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1021 atgagtgagc taactcacat taattgcgt
//
