LOCUS       sequence-157096-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DsREDmonomer-based BiFC vector for transient expression of RC 
            (169-225) in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBRC169
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..86
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      310..562
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(613..635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(648..654,655..672,673..678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    693..714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(831..848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggagaggac aggcttcttg agatccttca acaattacca acaacaacaa acaacaaaca
       61 acattacaat tactatttac aattacagtc gactctagag gatccatgga cggcggccac
      121 tacacctgcg acttcaagac cgtttacaag gccaagaagc ccgtgcagct gcccggcaac
      181 cactacgtgg actccaagct ggacatcacc aaccacaacg aggactacac cgtggtggag
      241 cagtacgagc acgccgaggc ccgccactcc ggctcccagc tgtacaagta aagcggccgc
      301 ccggctgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
      361 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
      421 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
      481 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
      541 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagaatt cgtaatcatg
      601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gac
//
