LOCUS       sequence-157097-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DsREDmonomer-based BiFC vector for transient expression of RC 
            (169-225) in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBRC169
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    194..249
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(269..614)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(306..326)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(674..691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(674..690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(683..705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(899..918)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgggagccg gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg tgtagtcctc
       61 gttgtggttg gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca gctgcacggg
      121 cttcttggcc ttgtaaacgg tcttgaagtc gcaggtgtag tggccgccgt ccatggatcc
      181 tctagagtcg actgtaattg taaatagtaa ttgtaatgtt gtttgttgtt tgttgttgtt
      241 ggtaattgtt gaaggatctc aagaagcctg tcctctccaa atgaaatgaa cttccttata
      301 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
      361 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgt
      421 tcctcgtggg tgggggtcca tctttgggac cactgtcggt agaggcatct tgaacgatag
      481 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttct actgtccttt
      541 cgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
      601 gttgaaaagt ctcaatagcc ctctggtctt ctgagggggg atcctctaga gcctgcaggc
      661 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      721 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      841 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      901 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctga
//
