LOCUS       sequence-157111-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector encoding non-targeting shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pEN_TmiRc3_shNT
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      227..313
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      405..432
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     440..463
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agcatggatc
      181 tcggggacgt ctaactacta agcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      241 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      301 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg
      361 gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac
      421 ggatggcctt tttgcgtttc tacaaactct tcctgttagt tagttactta agctcgggcc
      481 ccaaataatg attttatttt gactgatag
//
