LOCUS       sequence-157119-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #1-RFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(82..116)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(322..341)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcgctactc gccatatgta
       61 tatctccttc ttaaaagatc cgctagcatt atacctagga ctgagctagc tgtcaaagat
      121 ctcatgaatt cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
      181 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc atgatctgat
      241 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
      301 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      361 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      421 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      481 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      541 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      601 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      661 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      721 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      781 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      841 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      901 ctgggtgtgg cggaccgcta tcaggacata gcg
//
