LOCUS       sequence-157120-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #1-RFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      162..233
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      249..276
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(304..321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(518..537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
       61 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      121 ggtgcttaag gatccaaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc
      181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggga tatattccgc
      301 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
      361 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
      421 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
      481 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      541 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
      601 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
      661 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
      721 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
      781 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
      841 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
//
