LOCUS       sequence-157132-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #4-RFP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      155..226
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      242..269
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(297..314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(511..530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
       61 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      121 aaggatccaa actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa
      181 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      241 tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggatatattc cgcttcctcg
      301 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
      361 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
      421 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
      481 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
      541 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
      601 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
      661 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
      781 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
      841 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
      901 cttcgaaaaa ccgccctgc
//
