LOCUS       sequence-157133-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression vector for sfGFP in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Fujii Y, Kodama Y
  TITLE     In planta comparative analysis of improved green fluorescent 
            proteins with reference to fluorescence intensity and bimolecular 
            fluorescence complementation ability.
  JOURNAL   Plant Biotech. 2015; 32(1): 81-87.
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotech."; date: "2015"; volume: " 32(1)"; pages: " 81-87"
COMMENT     Sequence Label: 35S-sfGFP-nosT
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    33..88
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(108..110,111..113,114..827)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(153..174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     761..782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttggagagg acaggcttct tgagatcctt caacaattac caacaacaac aaacaacaaa
       61 caacattaca attactattt acaattacag tcgactctag aggatccatg gtgagcaagg
      121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      181 gccacaagtt cagcgtgaga ggcgagggcg agggcgatgc caccaacggc aagctgaccc
      241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg
      421 gcacttacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      541 acttcaacag ccacaacgtc tatatcactg ccgacaagca gaagaacggc atcaaggcca
      601 acttcaagat ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc
      661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      721 agtccgttct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcccgg
      841 ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
      901 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
      961 aatgcatgac gttatttatg agatgggttt ttatgattag agtcc
//
