LOCUS       sequence-157164-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura 
            T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, 
            Meyerson M
  TITLE     Allele-dependent variation in the relative cellular potency of 
            distinct EGFR inhibitors.
  JOURNAL   Cancer Biol Ther. 2007 May . 6(5):661-7.
  PUBMED    17495523
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Biol
            Ther. 2007 May . 6(5):661-7."
COMMENT     Sequence Label: pBabe EGFR insertionH
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..485
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    550..749
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             950..1366
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1296..1318
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1334..1350
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(1394..1465,1466..3328,3329..3397,3398..5029)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGS
                     QYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYH
                     AEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEK
                     GERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMH
                     LPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTV
                     ACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQ
                     NPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQI
                     SLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     primer_bind     complement(5066..5086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5071..5400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5251..5386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5410..6009
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5410..5429)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5906..5926
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6230..6699
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6829..6846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7000..7588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7080..7099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7748..8539,8540..8608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8609..8713)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
      121 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
      181 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
      241 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
      301 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
      361 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
      421 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
      481 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
      541 ccgggaggca agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac
      601 tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg
      661 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
      721 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
      781 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
      841 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
      901 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
      961 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     1021 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     1081 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     1141 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     1201 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     1261 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     1321 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt
     1381 gtggtggtac atcatgcgac cctccgggac ggccggggca gcgctcctgg cgctgctggc
     1441 tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag gcacgagtaa
     1501 caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga ggatgttcaa
     1561 taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga attatgatct
     1621 ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc tcaacacagt
     1681 ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact acgaaaattc
     1741 ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga aggagctgcc
     1801 catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca accctgccct
     1861 gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc tcagcaacat
     1921 gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa gctgtcccaa
     1981 tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa tcatctgtgc
     2041 ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc acaaccagtg
     2101 tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca aattccgaga
     2161 cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca ccacgtacca
     2221 gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga agaagtgtcc
     2281 ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg ccgacagcta
     2341 tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt gccgcaaagt
     2401 gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg ctacgaatat
     2461 taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc cggtggcatt
     2521 taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg atattctgaa
     2581 aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa acaggacgga
     2641 cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac atggtcagtt
     2701 ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc tcaaggagat
     2761 aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa atacaataaa
     2821 ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca acagaggtga
     2881 aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg agggctgctg
     2941 gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca gggaatgcgt
     3001 ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact ctgagtgcat
     3061 acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag gacggggacc
     3121 agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca agacctgccc
     3181 ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg ccggccatgt
     3241 gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc ttgaaggctg
     3301 tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg ccctcctctt
     3361 gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca tcgttcggaa
     3421 gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg
     3481 agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa
     3541 agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga
     3601 gaaagttaaa attcccgtcg ctatcaagga attaagagaa gcaacatctc cgaaagccaa
     3661 caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc accatgtgtg
     3721 ccgcctgctg ggcatctgcc tcacctccac cgtgcaactc atcacgcagc tcatgccctt
     3781 cggctgcctc ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct
     3841 caactggtgt gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca
     3901 ccgcgacctg gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga
     3961 ttttgggctg gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa
     4021 agtgcctatc aagtggatgg cattggaatc aattttacac agaatctata cccaccagag
     4081 tgatgtctgg agctacgggg tgaccgtttg ggagttgatg acctttggat ccaagccata
     4141 tgacggaatc cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca
     4201 gccacccata tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc
     4261 agatagtcgc ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc
     4321 ccagcgctac cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc
     4381 caacttctac cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga
     4441 gtacctcatc ccacagcagg gcttcttcag cagcccctcc acgtcacgga ctcccctcct
     4501 gagctctctg agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct
     4561 gcaaagctgt cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg
     4621 cgccttgact gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca
     4681 gtccgttccc aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct
     4741 gaaccccgcg cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa
     4801 ccccgagtat ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc
     4861 ccactgggcc cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga
     4921 cttctttccc aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc
     4981 agaataccta agggtcgcgc cacaaagcag tgaatttatt ggagcatgag atgtaggaat
     5041 tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5281 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5401 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5461 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5521 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5581 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5641 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     5701 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5761 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5821 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5881 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5941 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6001 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     6061 gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     6121 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     6181 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     6241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     6301 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     6361 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     6421 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     6481 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     6541 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     6601 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     6661 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     6721 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     6781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7441 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7681 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8701 taggggttcc gcgcacattt ccccgaaa
//
