LOCUS       sequence-157172-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episome vector for Phaeodactylum tricornutum (pBR322-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     pPtPBR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPtPBR11
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..211)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     misc_feature    212..715
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cgactctaga gtctgctctg tcatctttgc cttcgtttat cttgcctgct cattttttag
       61 cctattcttc gaagaaatca cattacttta tataatgtat aattcattat gtgataatgc
      121 caatcgctaa gaaaaaaaaa gagtcatccg ctaggtggaa aaaaaaaatg aaaatcatta
      181 ccgaggcata aaaaaatata gagtgtacta ggatcgcttg cctgtaactt acacgcgcct
      241 cgtatctttt aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt
      301 ttgtatttgg attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa
      361 aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta
      421 gacaagaaaa gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc
      481 acaggatttt cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc
      541 tgagagcagg aagagcaagg taaaaggtag tatttgttgg cgatccccct agagtctttt
      601 acatcttcgg aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta
      661 atttatatat ttatattaaa aaatttaaat tataattatt tttatagcac gtgattgaag
      721 acgagctagt gttattcctg actgtgaaac caaagcggag tgactgcaac taatgaaaat
      781 taaattttta aggaaggata gagactcc
//
