LOCUS       sequence-157173-        8710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura 
            T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, 
            Meyerson M
  TITLE     Allele-dependent variation in the relative cellular potency of 
            distinct EGFR inhibitors.
  JOURNAL   Cancer Biol Ther. 2007 May . 6(5):661-7.
  PUBMED    17495523
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Biol
            Ther. 2007 May . 6(5):661-7."
COMMENT     Sequence Label: pBabe EGFR Del1(T790M)
FEATURES             Location/Qualifiers
     source          1..8710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..492
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    557..756
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(1401..1472,1473..3335,3336..3404,3405..5018)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKTSPKANKE
                     ILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGCLLDYVREHKDNIGSQYLLNW
                     CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKV
                     PIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQ
                     PPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTD
                     SNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRN
                     GLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHN
                     QPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPD
                     YQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     primer_bind     complement(5055..5075)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5060..5389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5240..5375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5399..5998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5399..5418)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5895..5915
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6219..6688
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6818..6835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6989..7577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7069..7088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7737..8528,8529..8597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8598..8702)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca gcctgaatat gggccaaaca ggatatctgt ggtaagcagt
       61 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
      181 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
      241 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
      301 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
      361 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      421 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      481 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      541 cccaccaccg ggaggcaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      601 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      661 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
      721 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
      781 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
      841 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
      901 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga
     1381 tcccagtgtg gtggtacatc atgcgaccct ccgggacggc cggggcagcg ctcctggcgc
     1441 tgctggctgc gctctgcccg gcgagtcggg ctctggagga aaagaaagtt tgccaaggca
     1501 cgagtaacaa gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga
     1561 tgttcaataa ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt
     1621 atgatctttc cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca
     1681 acacagtgga gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg
     1741 aaaattccta tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg
     1801 agctgcccat gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc
     1861 ctgccctgtg caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca
     1921 gcaacatgtc gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct
     1981 gtcccaatgg gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca
     2041 tctgtgccca gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca
     2101 accagtgtgc tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat
     2161 tccgagacga agccacgtgc aaggacacct gccccccact catgctctac aaccccacca
     2221 cgtaccagat ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga
     2281 agtgtccccg taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg
     2341 acagctatga gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc
     2401 gcaaagtgtg taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta
     2461 cgaatattaa acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg
     2521 tggcatttag gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata
     2581 ttctgaaaac cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca
     2641 ggacggacct ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg
     2701 gtcagttttc tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca
     2761 aggagataag tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata
     2821 caataaactg gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca
     2881 gaggtgaaaa cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg
     2941 gctgctgggg cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg
     3001 aatgcgtgga caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg
     3061 agtgcataca gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac
     3121 ggggaccaga caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga
     3181 cctgcccggc aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg
     3241 gccatgtgtg ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg
     3301 aaggctgtcc aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc
     3361 tcctcttgct gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg
     3421 ttcggaagcg cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac
     3481 ccagtggaga agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa
     3541 agatcaaagt gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag
     3601 aaggtgagaa agttaaaatt cccgtcgcta tcaaaacatc tccgaaagcc aacaaggaaa
     3661 tcctcgatga agcctacgtg atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg
     3721 gcatctgcct cacctccacc gtgcaactca tcatgcagct catgcccttc ggctgcctcc
     3781 tggactatgt ccgggaacac aaagacaata ttggctccca gtacctgctc aactggtgtg
     3841 tgcagatcgc aaagggcatg aactacttgg aggaccgtcg cttggtgcac cgcgacctgg
     3901 cagccaggaa cgtactggtg aaaacaccgc agcatgtcaa gatcacagat tttgggctgg
     3961 ccaaactgct gggtgcggaa gagaaagaat accatgcaga aggaggcaaa gtgcctatca
     4021 agtggatggc attggaatca attttacaca gaatctatac ccaccagagt gatgtctgga
     4081 gctacggggt gaccgtttgg gagttgatga cctttggatc caagccatat gacggaatcc
     4141 ctgccagcga gatctcctcc atcctggaga aaggagaacg cctccctcag ccacccatat
     4201 gtaccatcga tgtctacatg atcatggtca agtgctggat gatagacgca gatagtcgcc
     4261 caaagttccg tgagttgatc atcgaattct ccaaaatggc ccgagacccc cagcgctacc
     4321 ttgtcattca gggggatgaa agaatgcatt tgccaagtcc tacagactcc aacttctacc
     4381 gtgccctgat ggatgaagaa gacatggacg acgtggtgga tgccgacgag tacctcatcc
     4441 cacagcaggg cttcttcagc agcccctcca cgtcacggac tcccctcctg agctctctga
     4501 gtgcaaccag caacaattcc accgtggctt gcattgatag aaatgggctg caaagctgtc
     4561 ccatcaagga agacagcttc ttgcagcgat acagctcaga ccccacaggc gccttgactg
     4621 aggacagcat agacgacacc ttcctcccag tgcctgaata cataaaccag tccgttccca
     4681 aaaggcccgc tggctctgtg cagaatcctg tctatcacaa tcagcctctg aaccccgcgc
     4741 ccagcagaga cccacactac caggaccccc acagcactgc agtgggcaac cccgagtatc
     4801 tcaacactgt ccagcccacc tgtgtcaaca gcacattcga cagccctgcc cactgggccc
     4861 agaaaggcag ccaccaaatt agcctggaca accctgacta ccagcaggac ttctttccca
     4921 aggaagccaa gccaaatggc atctttaagg gctccacagc tgaaaatgca gaatacctaa
     4981 gggtcgcgcc acaaagcagt gaatttattg gagcatgaga tgtaggaatt cgccagcaca
     5041 gtggtcgacc atgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5161 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5221 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5281 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5341 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat
     5401 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5461 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5521 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5581 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5641 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5701 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5761 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5821 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5881 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5941 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     6001 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa
     6061 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     6121 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     6181 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
     6241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
     6301 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     6361 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
     6421 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     6481 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     6541 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     6601 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     6661 ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt
     6721 ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg
     6781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7681 tccttttgcg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7741 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7801 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7861 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7921 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7981 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8041 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8101 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8161 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8221 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8281 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8341 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8401 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8461 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8521 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8581 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8701 cgcacatttc
//
