LOCUS       sequence-157175-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episome vector for Phaeodactylum tricornutum (pBR322-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  TITLE     pPtPBR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPtPBR2
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(335..709)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 tctagattcg ctaccttagg accgttatag ttacgtgaag acgagctagt gttattcctg
       61 actgtgaaac caaagcggag tgactgcaac taatgaaaat taaattttta aggaaggata
      121 gagactccca actgttcgtg caccatgaga gcaaggatgc ccatttgtgc cgcacgtccc
      181 tggttgagtt cgatagcacg cttctgaagc ttcttgtcct cactgaaagt gtcccagcca
      241 aagtcgaggt agttgttgcg gccgcatggg cccagatctc cgaggcctcg gacccgtcgg
      301 gccgcgtcgg accggcggtg ttggtcggcg tcggtcagtc ctgctcctcg gccacgaagt
      361 gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga
      421 tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact
      481 cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca
      541 cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt
      601 cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg
      661 cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg ggtaccctca
      721 gctagaatat tatcgatacc gtcgacttgg ttaatttttc gatt
//
