LOCUS       sequence-157187-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of murine TDG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, 
            Schuermann D, Klungland A, Schar P
  TITLE     Biochemical reconstitution of TET1-TDG-BER-dependent active DNA 
            demethylation reveals a highly coordinated mechanism.
  JOURNAL   Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
  PUBMED    26932196
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-2"; volume: "7:10806. doi"; pages: " 
            10.1038/ncomms10806"
COMMENT     Sequence Label: pET28-mTDGa.0
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agcatggacg cagaggccgc
      121 gcgcagctat tctctggagc aagttcaagc tttgtattca tttccatttc aacaaatgat
      181 ggcagaagtt cctaacatgg cagtcacgac tggacagcag gtgccagcag tagctcctaa
      241 catggcaacc gtgacagaac agcaggtgcc cgcagacgct cctgtccagg aacctgcacc
      301 agaagctcca aagagaagga aaaggaaacc cagagcagca gagccccagg aaccagtgga
      361 gcccaaaaaa cctgctacgt cgaagaaatc cggcaagtct acaaaatcaa aggaaaagca
      421 ggagaaaatc acagacgcgt ttaaagtgaa aaggaaagtg gaccgcttca acggcgtctc
      481 tgaagctgag cttctgacca agactcttcc tgacattttg accttcaatc tggatattgt
      541 gatcattggc attaacccgg gattaatggc tgcttacaaa ggacatcact accctgggcc
      601 tggaaatcac ttctggaagt gtctgttcat gtcggggctg agtgaggtgc agctgaatca
      661 catggatgac cacaccttac ccggcaagta cggcatcgga ttcaccaaca tggtggaacg
      721 gacgacgccg ggcagcaagg atctgtctag taaagagttc cgggaaggag ggcgcatcct
      781 ggtgcagaaa ctgcagaaat atcagccacg aatagcggtg tttaatggaa aatgtattta
      841 tgaaatt
//
