LOCUS       sequence-157188-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of murine TDG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, 
            Schuermann D, Klungland A, Schar P
  TITLE     Biochemical reconstitution of TET1-TDG-BER-dependent active DNA 
            demethylation reveals a highly coordinated mechanism.
  JOURNAL   Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
  PUBMED    26932196
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-2"; volume: "7:10806. doi"; pages: " 
            10.1038/ncomms10806"
COMMENT     Sequence Label: pET28-mTDGa.0
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ctaagcgtgg ctctcttctt cctgctctcg ggtcccacaa ttattaaaag
      121 aagggatctg ctctgcaaac gactgtgcca tccactgccc atttggaaca tcgccggggg
      181 tcgcttctcc tctcggctcc gcactgtggg ctgttagccc atttgaagca atgccacaag
      241 gttccccatt acatgggttt tccccatagg cgccgccgta agctgcctca tagcctggat
      301 catacttttc ttccttaaca gccatcttct ttgcgtcctc ttgcgcaagc tgcaggtcaa
      361 atgtatactg cacttcctga acgtcggtgt tgcgttcaat gcctttcagc tggtctctca
      421 agtccttcag cttaatgtag taatgaactt tgtcctgggc ccggggaaac tgagcacatc
      481 tggcgctgga cgacggcatg acgtagcaca gagtttctgt gtctgggatc ttgtggggtt
      541 gaagcccaaa ttccaagttc ttaaccttta ctccaaaaac ttctttactg aaaatttcat
      601 aaatacattt tccattaaac accgctattc gtggctgata tttctgcagt ttctgcacca
      661 ggatgcgccc tccttcccgg aactctttac tagacagatc cttgctgccc ggcgtcgtcc
      721 gttccaccat gttggtgaat ccgatgccgt acttgccggg taaggtgtgg tcatccatgt
      781 gattcagctg cacctcactc agcc
//
