LOCUS       sequence-157193-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of catalytically dead murine TDG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, 
            Schuermann D, Klungland A, Schar P
  TITLE     Biochemical reconstitution of TET1-TDG-BER-dependent active DNA 
            demethylation reveals a highly coordinated mechanism.
  JOURNAL   Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
  PUBMED    26932196
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-2"; volume: "7:10806. doi"; pages: " 
            10.1038/ncomms10806"
COMMENT     Sequence Label: pET28-mTDGa.1
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacctaagcg tggctctctt cttcctgctc tcgggtccca caattattaa aagaagggat
      121 ctgctctgca aacgactgtg ccatccactg cccatttgga acatcgccgg gggtcgcttc
      181 tcctctcggc tccgcactgt gggctgttag cccatttgaa gcaatgccac aaggttcccc
      241 attacatggg ttttccccat aggcgccgcc gtaagctgcc tcatagcctg gatcatactt
      301 ttcttcctta acagccatct tctttgcgtc ctcttgcgca agctgcaggt caaatgtata
      361 ctgcacttcc tgaacgtcgg tgttgcgttc aatgcctttc agctggtctc tcaagtcctt
      421 cagcttaatg tagtaatgaa ctttgtcctg ggcccgggga aactgagcac atctggcgct
      481 ggacgacggc atgacgtagc acagagtttc tgtgtctggg atcttgtggg gttgaagccc
      541 aaattccaag ttcttaacct ttactccaaa aacttcttta ctgaaaattt cataaataca
      601 ttttccatta aacaccgcta ttcgtggctg atatttctgc agtttctgca ccaggatgcg
      661 ccctccttcc cggaactctt tactagacag atccttgctg cccggcgtcg tccgttccac
      721 catgttggtg aatccgatgc cgtacttgcc gggtaaggtg tggtcatcca tgtgattcag
      781 ctgcacctca ctcagcc
//
