LOCUS       sequence-157205-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a CRISPR sgRNA that allows for mutation of the surface 
            antigen SAG1 in Toxoplasma gondii and confers resistance to 
            pyrimethamine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pU6-SAG1-DHFR
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgggaggccg ttgttgatgc cgatgcccct cttgggggtc atcgcgacga ccagacacac
       61 cggtttctgc atcttcccag acacgacaac gccccgtaga gcagaaacgc actactaaag
      121 cgaaacttca cccgtccctg ctgcactcag agcagtgctc cgcactgccg tgtggtaaaa
      181 tgaaaaggtt ctacgagaca cgcgtctccg gatcgacaag cgaaggatct gcacacctgc
      241 tctcgatgtc gaacaaagca cggaggagag acggaaagtg cttacatcga acacggttat
      301 caaacccgag aaaaagaaac gaacagaaga aaaaggaaac ctccgcatac ttttaaagaa
      361 tgaagttccc cgattttccc aaaaatggcg tcattttcgc gcacggcagt cagataacag
      421 gtgtagcggc tgcccaccaa cagagacggc gcggccgaca ggacgctact gggactgcga
      481 acagcagcaa gatcggatct tccgcgggcg ggtttgaatg caaggtttcg tgctgtcgaa
      541 aaagagaatg gaacttaatg tccacgtagt tcgcgcatga aaagcgtcct accactcaaa
      601 aaagacaaaa accgcactcg cggcgttgaa tgtgcggggg aacggatggc tctctttttt
      661 ggaaagcgaa tgcaaaaaga caagacgcag acgcatacaa cgttagtccc ctggcacgag
      721 agatagaggt gctgggggcc attgcggtgt cgtggattta ccagtcatgg acgagatcgt
      781 tccggagcct gtctttcctg aaagtctgaa aaaattgcgg gaaagtcacg catatggaac
      841 agcaccgggg cgggacagag ttgtctcgac aacgaatgac acacagactc
//
