LOCUS       sequence-157207-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #5-RFP
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(91..125)
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     primer_bind     complement(331..350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     941..960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cggaaccttc catacgaact ttgaaacgca tgaactcttt gataacgtct tcgctactcg
       61 ccatatgtat atctccttct taaaagatcc gctagcattg tacctaggac tgagctagcc
      121 gtaaaagatc tcatgaattc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
      181 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
      241 tgatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
      301 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      361 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      421 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      481 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      541 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      601 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      661 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      721 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      781 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
      841 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
      901 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
      961 gctgaaa
//
