LOCUS       sequence-157208-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #5-RFP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      154..225
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      241..268
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(296..313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(510..529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
       61 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
      121 aggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa
      181 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      241 ggctcacctt cgggtgggcc tttctgcgtt tatacctagg gatatattcc gcttcctcgc
      301 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
      361 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
      421 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
      481 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
      541 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
      601 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
      661 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
      721 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
      781 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
      841 ggtgactgcg ctcctccaag cc
//
