LOCUS       sequence-157210-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #6-RFP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      146..217
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      233..260
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(288..305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(502..521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
       61 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taaggatcca
      121 aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      181 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      241 ttcgggtggg cctttctgcg tttataccta gggatatatt ccgcttcctc gctcactgac
      301 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
      361 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
      421 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
      481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
      541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
      601 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
      661 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      721 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
      781 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
      841 cgctcctc
//
