LOCUS       sequence-157211-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BiFC vector for transient expression of frGN155-MXMT in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Fujii Y, Kodama Y
  TITLE     In planta comparative analysis of improved green fluorescent 
            proteins with reference to fluorescence intensity and bimolecular 
            fluorescence complementation ability.
  JOURNAL   Plant Biotech. 2015; 32(1): 81-87.
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotech."; date: "2015"; volume: " 32(1)"; pages: " 81-87"
COMMENT     Sequence Label: 35S-frGN155-MXMT-NosT
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    33..88
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(108..110,111..113,114..572)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(153..174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(414..433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttggagagg acaggcttct tgagatcctt caacaattac caacaacaac aaacaacaaa
       61 caacattaca attactattt acaattacag tcgactctag aggatccatg gtgagcaagg
      121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg
      421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      541 actacaacag ccacaacgtc tatatcactg ccctgtacaa gatggagctc caagaagtcc
      601 tgcatatgaa tgaaggtgaa ggcgatacaa gctacgccaa gaatgcatcc tacaatctgg
      661 ctcttgccaa ggtgaaacct ttccttgaac aatgcatacg agaattgttg cgggccaact
      721 tgcccaacat caacaagtgc attaaagttg cggatttggg atgcgcttct ggaccaaaca
      781 cacttttaac agtgcgggac attgtgcaaa gtattgacaa agttggccag gaagagaaga
      841 atgaattaga acgtcccacc attcagattt ttctgaatga tcttttccaa aatgatttca
      901 attcggtttt caagttgctg ccaagcttct accgcaaact cgagaaagaa aatggacgca
      961 agataggatc gtgcctaata agcgcaatgc ctggctcttt ctacggcaga ctcttccccg
     1021 aggagtccat gcattttttg cactct
//
