LOCUS       sequence-157217-       11441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-tdiRFP-2A-LBR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto 
            T, Ochiai H
  TITLE     Role of dynamic nuclear deformation on genomic architecture 
            reorganization.
  JOURNAL   PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep.
  PUBMED    31509522
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2019-09-11"; volume: "15(9):e1007289. doi"; pages: " 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep"
COMMENT     Sequence Label: pLR5-CAG-NLS-tdiRFP670-2A-LBR
FEATURES             Location/Qualifiers
     source          1..11441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1031..1410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1413..1688
                     /label=chicken beta-actin promoter
     intron          1689..2697
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2621..2643
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        2795..2813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2893..2902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4885..4938
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     intron          6888..7117
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7100..7119
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7190..7763
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7357..7374)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7584..7603
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7799..8602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7862..7881)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8472..8491
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8732..8853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8769..8788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   9140..9202
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9253..9273)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9253..9271)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9292..9308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9292..9308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9305..9327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9316..9332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9340..9346,9347..9364,9365..9370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9385..9406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9523..9540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9694..10282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9774..9793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10453..11244,11245..11313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11076..11095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11314..11418)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagctgc ttcgcgatgt acgggccaga
     1021 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     1081 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1141 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1201 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1261 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1321 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1381 atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac
     1441 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     1501 ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
     1561 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
     1621 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
     1681 ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc
     1741 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1801 ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc
     1861 gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt
     1921 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg
     1981 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc
     2041 cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt
     2101 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct
     2161 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg
     2221 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     2281 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc
     2341 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     2401 gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca
     2461 ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     2521 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg
     2581 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     2641 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     2701 ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagagct ctctggctaa
     2761 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     2821 aagcttggta tcgacctcgg atcgtcgcta gcgatatcgg cgcgccagca tttaaatctg
     2881 tacagaccgg tcgccaccat gggcccaaaa aagaaaagaa aagttgaatt catggccaga
     2941 aaagtggacc tgaccagttg tgaccgagag ccgatacaca ttcctggctc tatccagcca
     3001 tgtgggtgtt tgctggcctg cgatgcccag gcagtcagga ttactcgaat taccgaaaat
     3061 gcgggtgcct tctttggccg tgaaacgccc cgtgtcggcg aactcttagc cgattatttc
     3121 ggagaaaccg aggctcacgc acttaggaac gcactggcgc aatcaagcga tcccaaacgc
     3181 cctgctctca tctttgggtg gagagatgga ctgacaggcc gtactttcga cataagcctt
     3241 caccggcatg acggaaccag tataattgag tttgaaccgg ccgccgctga gcaagcagac
     3301 aacccccttc gcctgaccag acaaattatc gcccgcacta aggagctgaa atcgctggaa
     3361 gaaatggccg ctcgtgttcc caggtatctt caggcaatgc tgggttatca tagggtaatg
     3421 ctctacagat tcgccgacga tggctctggt atggtaatag gggaggccaa gcgatccgac
     3481 ctggagtctt ttctggggca gcatttcccc gcatctctag taccccagca ggcaaggctt
     3541 ttgtacctca agaatgccat ccgcgtggta tcggactccc gcggtattag tagcaggatc
     3601 gttccagaac acgacgcctc tggagctgcg ctggatctgt ctttcgctca cctccgatct
     3661 atcagccctt gtcatttaga gtttctgcga aatatggggg tcagcgcaag tatgtccctg
     3721 agcataataa tcgatggaac actatggggc ctaataattt gccatcatta cgaacctaga
     3781 gcagtaccaa tggcacagcg agtggccgcc gaaatgttcg ccgatttttt gtctcttcat
     3841 tttactgccg ctcaccatca gcgcggccac gggactggct cgacaggttc tggcagtagt
     3901 gggactgctt catcagaaga taataacatg gccatggcca gaaaagtgga cctgaccagt
     3961 tgtgaccgag agccgataca cattcctggc tctatccagc catgtgggtg tttgctggcc
     4021 tgcgatgccc aggcagtcag gattactcga attaccgaaa atgcgggtgc cttctttggc
     4081 cgtgaaacgc cccgtgtcgg cgaactctta gccgattatt tcggagaaac cgaggctcac
     4141 gcacttagga acgcactggc gcaatcaagc gatcccaaac gccctgctct catctttggg
     4201 tggagagatg gactgacagg ccgtactttc gacataagcc ttcaccggca tgacggaacc
     4261 agtataattg agtttgaacc ggccgccgct gagcaagcag acaaccccct tcgcctgacc
     4321 agacaaatta tcgcccgcac taaggagctg aaatcgctgg aagaaatggc cgctcgtgtt
     4381 cccaggtatc ttcaggcaat gctgggttat catagggtaa tgctctacag attcgccgac
     4441 gatggctctg gtatggtaat aggggaggcc aagcgatccg acctggagtc ttttctgggg
     4501 cagcatttcc ccgcatctct agtaccccag caggcaaggc ttttgtacct caagaatgcc
     4561 atccgcgtgg tatcggactc ccgcggtatt agtagcagga tcgttccaga acacgacgcc
     4621 tctggagctg cgctggatct gtctttcgct cacctccgat ctatcagccc ttgtcattta
     4681 gagtttctgc gaaatatggg ggtcagcgca agtatgtccc tgagcataat aatcgatgga
     4741 acactatggg gcctaataat ttgccatcat tacgaaccta gagcagtacc aatggcacag
     4801 cgagtggccg ccgaaatgtt cgccgatttt ttgtctcttc attttactgc cgctcaccat
     4861 cagcgcggat ccgcgtttaa actcgaggga agaggtagcc tgctaacttg cggggacgta
     4921 gaggaaaatc ctggtcccat gccaagtagg aagtttgttg agggtgaagt ggtcagaggc
     4981 cgatggcctg ggagctccct ttattatgaa gtagagattc tgagccacga caacaaatcc
     5041 cagctctaca ctgtaaaata caaagatggc accgaactgg aactgaagga gagtgatatc
     5101 aagcctttaa aatcctttaa gcaaagaaaa agtggctcga tttccagctc tccctctcga
     5161 cgccgtggca gccgcagccg cagccgctca agatcacgtt cccgatcccc gggcagagca
     5221 ccgaaaggct ccagaagatc cgtctctgct tcccatgagg gtgacgtgaa ggagaagaag
     5281 gagaaggaga tgaggaggga aattctgcaa gttaagctga ctccgcttgt gttgaagccg
     5341 ttcggaaaca gcgtcagcgt gtacaacggg gagcccgaac acatggagaa gaacgctacg
     5401 ccgtataaag acaagcagga aagaatcatt ttgtcaacag aagacagata tatagttaca
     5461 cagtacagcc ttcgtcccag gagagaagag gtcaaagcca aagaaataga gtctgaggaa
     5521 caaaaccttg tcaccaaagg acccgcacct ttgggaacct ttcaagtgac cactccacag
     5581 aggaaggact tggagtttgg aggagtacct ggtgcggtcc tcatcatgct gggcctgccc
     5641 gcctgcgtcc tcttgctgct gctgcagtgc aggcagaagg acccggggct gctccacttc
     5701 cctccacctc tgccggctct ccacgagctg tgggaaccca gggtgtgcgg cgtttacctc
     5761 ctctggtttt tcgttcaagc tctcttccac cttctaccag ttgggaaggt tgcagaagga
     5821 acacctcttg ttgatggaag aagactccag tatagactga atggattgta tgccttcatt
     5881 ctgacatctg cagccctggg agcagctgtc ttctggggtg ttgagctgtg ctacctgtac
     5941 actcacttcc tgcagttggc actggcagcc accgggttct ccgtgctcct gagtgcttac
     6001 ctctatgttc gctctctgcg agccccgagg gaggagctgt cgccagccag ttccggaaat
     6061 gctgtctacg acttcttcat tggccgagag ttaaacccgc gacttggtgc tttcgatctc
     6121 aagttctttt gtgagttgcg ccctggattg attggatggg tggttattaa cttagtgatg
     6181 cttctgatgg aaatgaaaat tcaggagcga gctgctccgt ccttggcaat gatcttggtt
     6241 aacagtttcc agctcttata tgtggtggac gccctctgga atgaggaagc actgctgacc
     6301 tccatggaca tcatgcacga cggctttggc ttcatgctgg cctttgggga tttagtgtgg
     6361 gttccattta cctacagcct ccaggccttt tacctggtca gccatcccca tgacttgtcc
     6421 tggccattgg cttctgtcat cattgctctg aaattgtgtg gatatgtaat cttccgttgt
     6481 gcaaactctc agaaaaatgc attccggaag aatcccactg atccaaagct tgcacatttg
     6541 aagaccattc atacttccac ggggaaaagt ctgcttgttt ccggatggtg gggctttgtt
     6601 cgccatccca attacttggg tgacctcatc atggctctgg cgtggtccct cccatgtggt
     6661 ttcaaccacc tcctgccata cttctacatt atctacttca ccgccttgct tatccatcga
     6721 gaggcccggg atgaacacca gtgcaggagg aagtacggcc tggcctggga gaagtactgc
     6781 cagcgagtgc cctaccgcat attcccctac atttactgag cggccgcata gataactgat
     6841 ccagtgtgct ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct
     6901 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
     6961 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa
     7021 gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg
     7081 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     7141 caggtcgagc atgcatctag ggcggccaat tccgcccctc tccccccccc ccttttccct
     7201 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     7261 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     7321 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     7381 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     7441 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     7501 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     7561 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     7621 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     7681 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     7741 tcctttgaaa aacacgatga taagcttgcc acaacccggg ataattcctg cagccaatat
     7801 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7861 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7921 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7981 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     8041 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     8101 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     8161 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     8221 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     8281 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     8341 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     8401 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     8461 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     8521 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     8581 ccttcttgac gagttcttct gaggggatca attctctaga taactgatca taatcagcca
     8641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     8701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     8761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8821 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gagtgcattc tagttgtggt
     8881 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8941 ttggcgtaat ctcgacatac tagttaaaag ttttgttact ttatagaaga aattttgagt
     9001 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     9061 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     9121 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     9181 caatatgatt atctttctag ggttaatcta gtatacgcgt atgcggccgc ttaattaatc
     9241 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     9301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    10141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11401 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
