LOCUS       sequence-157229-        8162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rOpto-rtrkB (light-activated receptor tyrosine kinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H
  TITLE     A Phytochrome Sensory Domain Permits Receptor Activation by Red 
            Light.
  JOURNAL   Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 
            10.1002/anie.201601736. Epub 2016 Apr 21.
  PUBMED    27101018
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201601736"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2016-05-17- 17"; volume: "55"; issue: "21"; pages: "6339-42"
COMMENT     Sequence Label: rOpto-rtrkB_397
FEATURES             Location/Qualifiers
     source          1..8162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      935..944
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             941..982
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             3653..3679
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3756..3773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3762..3986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4032..4460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4119..4138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4329..4350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4469..4489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4474..4803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4654..4789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4716..4735
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4870..5664
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4924..4943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5534..5553
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5838..5959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5875..5894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5929..5948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6008..6024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6008..6024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6021..6043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6032..6048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6056..6062,6063..6080,6081..6086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6101..6122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6239..6256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6410..6995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6490..6509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7166..7957,7958..8026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7789..7808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8027..8131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgcggccacc atggggagta gcaagagcaa
      961 gcctaaggac cccagccagc gcctcgacgt caccggaaag tttggcatga aaggcccagc
     1021 ttccgtcatc agcaacgacg atgactctgc cagccctctc caccacatct ccaacgggag
     1081 caacactccg tcttcttcgg agggcgggcc cgatgctgtc atcattggga tgaccaagat
     1141 ccctgtcatt gaaaaccccc agtacttcgg tatcaccaac agccagctca agccggacac
     1201 atttgttcag cacatcaaga gacacaacat cgttctgaag agggagcttg gagaaggagc
     1261 ctttgggaaa gttttcctag cggagtgcta taacctctgc cccgagcagg ataagatcct
     1321 ggtggccgtg aagacgctga aggacgccag cgacaatgct cgcaaggact ttcatcgcga
     1381 agccgagctg ctgaccaacc tccagcacga gcacattgtc aagttctacg gtgtctgtgt
     1441 ggagggcgac ccactcatca tggtctttga gtacatgaag cacggggacc tcaacaagtt
     1501 ccttagggca cacgggccag atgcagtgct gatggcagag ggtaacccgc ccaccgagct
     1561 gacgcagtcg cagatgctgc acatcgctca gcaaatcgca gcaggcatgg tctacctggc
     1621 atcccaacac ttcgtgcacc gagacctggc cacccggaac tgcttggtag gagagaacct
     1681 gctggtgaaa attggggact tcgggatgtc ccgggatgta tacagcaccg actactaccg
     1741 ggttggtggc cacacaatgt tgcccatccg atggatgcct ccagagagca tcatgtacag
     1801 gaaattcacc accgagagtg acgtctggag cctgggagtt gtgttgtggg agatcttcac
     1861 ctacggcaag cagccctggt atcagctatc aaacaacgag gtgatagaat gcatcaccca
     1921 gggcagagtc cttcagcggc ctcgcacgtg tccccaggag gtgtacgagc tgatgctggg
     1981 atgctggcag cgggaaccac acacaaggaa gaacatcaag aacatccaca cactccttca
     2041 gaacttggcg aaggcgtcgc ccgtctacct ggacatccta ggcaccggtg gagcaactac
     2101 tgttcaactg tctgatcaat ctctgcgtca actggaaact ctggctatcc acaccgcgca
     2161 tctgatccag ccgcacggtc tggtagtcgt cctgcaagaa ccggacctga ccatcagcca
     2221 gatctctgcg aactgtaccg gtatcctggg ccgtagcccg gaagatctgc tgggtcgtac
     2281 tctgggcgag gtattcgatt cttttcagat tgatccgatc cagtctcgtc tgaccgcagg
     2341 tcagatttcc agcctgaacc cgtccaagct gtgggcgcgt gttatgggtg acgactttgt
     2401 tattttcgac ggcgtatttc atcgtaactc tgatggcctg ctggtttgcg agctggagcc
     2461 ggcctacact agcgacaacc tgcctttcct gggtttctac catatggcaa acgcggcact
     2521 gaaccgtctg cgtcagcaag ctaacctgcg cgacttctac gacgttatcg ttgaggaagt
     2581 gcgccgcatg acgggtttcg accgcgtcat gctgtaccgt tttgatgaaa acaaccacgg
     2641 tgacgtaatc gcggaggata agcgtgacga catggagccg tatctgggtc tgcactaccc
     2701 ggaaagcgac attcctcagc cggcacgtcg cctgttcatt cacaacccga tccgtgttat
     2761 tccggacgtt tacggcgttg ctgttccgct gactccggcc gttaatccgt ctactaaccg
     2821 tgcagttgac ctgaccgaat ccatcctgcg ttccgcatac cattgccacc tgacctatct
     2881 gaagaacatg ggcgttggtg ctagcctgac gatctctctg attaaagatg gtcacctgtg
     2941 gggtctgatc gcttgccatc accagacccc gaaagtaatc cctttcgaac tgcgtaaagc
     3001 ctgcgaattc ttcggtcgtg tggtgttctc taatatctcc gcgcaagaag acaccgagac
     3061 ttttgactac cgcgtacagc tggcggagca tgaagcggtt ctgctggaca aaatgaccac
     3121 cgcggcagac ttcgtggagg gcctgactaa ccacccagac cgtctgctgg gcctgaccgg
     3181 cagccaaggc gctgcgattt gtttcggcga gaaactgatt ctggtgggcg aaaccccaga
     3241 cgaaaaggcg gtgcaatacc tgctgcaatg gctggagaat cgcgaagtgc aggacgtttt
     3301 cttcactagc tctctgtctc agatctatcc ggatgcggtt aacttcaaaa gcgtggcgtc
     3361 cggcctgctg gctatcccga tcgcccgtca taactttctg ctgtggttcc gcccggaggt
     3421 tctgcagacc gttaattggg gtggtgatcc gaatcacgca tacgaagcaa cccaagaaga
     3481 tggtaagatc gaactgcatc cgcgtcagtc cttcgatctg tggaaagaaa ttgttcgcct
     3541 gcagagcctg ccgtggcaga gcgttgagat ccagtctgcc ctggctctga agaaagcaat
     3601 cgtgaacctg attctgcgcc aagctgaaga acccggtgga tccggagtcg actatccgta
     3661 cgacgtacca gactacgcac tcgactaaga attccaccac actggactag tggatccgag
     3721 ctcggtacca agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca
     3781 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3841 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3901 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     3961 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4021 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4081 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4381 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4441 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4501 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4561 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4621 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4681 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4741 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4801 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4861 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5341 ctggacgaag aacatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5521 tatcaggaca tagcgttggc tacccgtgat attgctgaag aacttggcgg cgaatgggct
     5581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5701 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5761 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5821 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5941 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tgcgcttcct
     6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     6961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
     7321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8161 tc
//
