LOCUS       sequence-157237-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            H129N nuclease-dead mutant of human MRE11. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-MRE11-H129N
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagacgcgtc tcgagggtac cttatcttct atttcttctt aaagaactag tgttcataaa
       61 aggatcatca tcatcatcct cacttgattc aaaatcaacc cctttcgata cttgactctg
      121 ggacatgatt ttgctggatg atgtgctgga ccacctttga tctgtctttg aagtggtagg
      181 aaaaatgtct tcttccacat ctgattcatc tacctcaatc acctctgaat aattcttagt
      241 agtgacattt cgggaaggct gctgtcttgt agatttaaag gcatctataa tagacatatt
      301 tctagatgct gacacagcag tctttgagtt cctgctacgg gtagaagtct ccagaccagt
      361 gtctgctctt cctctttgag accctcctct cgatgctgaa ttctgccctc ttccacctct
      421 tcgacctctt cctcggcctc ttcctttgtt ggttgctgct gagatgctat catcagagtc
      481 attagccatc tgttctgcta aatctatact cataaggtca tcagcactaa aggcagaagc
      541 agactcctct gactgagatc tgagtgctct ggccctggtc atagcctcac ggacttcatc
      601 atcttcttca ttagtatttt tttgtctggt ttctctgaaa cgacgtacct cctcatcgat
      661 tttgtcttcg agggcatcaa tgtgacgttc tttaagaaat cgctgtgttt tttccaactg
      721 gtatttcact aattcctcaa tggcatcttt ctcctccttg tccacaaatt cttgtactgc
      781 ttcacccatc cctctttctg ttagcagtga gagctgcaca ttcttctctg cggtttgaaa
      841 gtactgtttt acaagatctt ctaccctta
//
