LOCUS       sequence-157243-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BiFC vector for transient expression of psGN155-MXMT in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Fujii Y, Kodama Y
  TITLE     In planta comparative analysis of improved green fluorescent 
            proteins with reference to fluorescence intensity and bimolecular 
            fluorescence complementation ability.
  JOURNAL   Plant Biotech. 2015; 32(1): 81-87.
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotech."; date: "2015"; volume: " 32(1)"; pages: " 81-87"
COMMENT     Sequence Label: 35S-psGN155-MXMT-NosT
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..78
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(98..100,101..103,104..562)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(143..164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(404..423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaggcttct tgagatcctt caacaattac caacaacaac aaacaacaaa caacattaca
       61 attactattt acaattacag tcgactctag aggatccatg gtgagcaagg gcgaggagct
      121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
      301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      541 ccacaacgtc tatatcatgg ccctgtacaa gatggagctc caagaagtcc tgcatatgaa
      601 tgaaggtgaa ggcgatacaa gctacgccaa gaatgcatcc tacaatctgg ctcttgccaa
      661 ggtgaaacct ttccttgaac aatgcatacg agaattgttg cgggccaact tgcccaacat
      721 caacaagtgc attaaagttg cggatttggg atgcgcttct ggaccaaaca cacttttaac
      781 agtgcgggac attgtgcaaa gtattgacaa agttggccag gaagagaaga atgaattaga
      841 acgtcccacc attcagattt ttctgaatga tcttttccaa aatgatttca attcggtttt
      901 caagttgctg ccaagcttct accgcaaact cgagaaagaa aatggacgca agataggatc
      961 gtgcctaata agcgcaatgc ctggctcttt ctacggcaga ctcttccccg aggagtccat
     1021 gcattttt
//
