LOCUS       sequence-157253-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: const #7-RFP
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      159..230
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      246..273
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(301..318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(515..534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
       61 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      121 gcttaaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
      181 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      241 acactggctc accttcgggt gggcctttct gcgtttatac ctagggatat attccgcttc
      301 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
      361 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
      421 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
      481 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
      541 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
      601 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
      661 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
      721 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
      781 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
      841 gttttggtga ctgcgctcct ccaagccagt tacctc
//
