LOCUS       sequence-157255-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Citrine fluorescent protein with a 
            peroxisome localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y
  TITLE     Cold-induced organelle relocation in the liverwort Marchantia 
            polymorpha L.
  JOURNAL   Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.
  PUBMED    23421791
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2013 Feb 20. doi: 10.1111/pce.12085."
COMMENT     Sequence Label: pDONR207-Citrine-PTS1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..186
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(200..451)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(242..263)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     591..610
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
       61 cacgggccag agctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct
      121 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
      181 ctgggtctca caacttggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      241 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      301 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      361 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      421 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
      481 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      541 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      601 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      661 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc
      721 cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      781 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      841 ggccgtttac gtc
//
