LOCUS       sequence-157260-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vectors for expression of Citrine fluorescent protein in M. 
            polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Tsuboyama S, Kodama Y
  TITLE     AgarTrap: a simplified Agrobacterium-mediated transformation method 
            for sporelings of the liverwort Marchantia polymorpha L.
  JOURNAL   Plant Cell Physiol. 2014 Jan;55(1):229-36. doi: 10.1093/pcp/pct168. 
            Epub 2013 Nov 19.
  PUBMED    24259681
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/pcp/pct168"; journalName: "Plant Cell Physiol"; date: 
            "2014-01"; volume: "55"; issue: "1"; pages: "229-36"
COMMENT     Sequence Label: pMpGWB103-Citrine
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..35
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(40..750,751..753,754..756))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     431..450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(759..783)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgttatca accactttgt acaagaaagc tgggtctcac ttgtacagct cgtccatgcc
       61 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      121 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg
      181 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      241 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      301 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      361 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      421 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      481 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
      541 gaagcacatc aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt
      601 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      661 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      721 caccccggtg aacagctcct cgcccttgct caccatggag cctgcttttt tgtacaaact
      781 tgttgataac tctagtcaac ctttctgcag gcacatcaat actgttgagg caacacccat
      841 agaagcacgg aaccaaggag caccgacgtc catagcacac agccaacctc gctgaccttc
      901 gactcctctt caagtcaagg atagacaaat ctatcatgca
//
