LOCUS       sequence-157262-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vectors for expression of Citrine fluorescent protein with a 
            nuclear localization signal in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y
  TITLE     Cold-induced organelle relocation in the liverwort Marchantia 
            polymorpha L.
  JOURNAL   Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.
  PUBMED    23421791
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2013 Feb 20. doi: 10.1111/pce.12085."
COMMENT     Sequence Label: pMpGWB102-Citrine-NLS
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..33
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(38..58)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(62..772,773..775,776..778))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(104..125)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     453..472
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     712..733
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(781..805)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctgttatcac cactttgtac aagaaagctg ggtctcacac cttgcgcttc ttcttaggag
       61 gcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      121 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt
      181 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      241 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
      301 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      361 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      421 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      481 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      541 tcatgtggtc ggggtagcgg gcgaagcaca tcaggccgta gccgaaggtg gtcacgaggg
      601 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      661 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      721 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
      781 agcctgcttt tttgtacaaa cttgttgata actctagagt cccccgtgtt ctctcc
//
