LOCUS       sequence-157266-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Vpr binding mutant EPN-01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01-(Delta-LF)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(221..250)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gggcttcatg atgtccccat aatttttggc agagggaaaa agatctgcta gcaagcttct
       61 cgagtcactg agaagacggg tcgttaccgg ccgcagaacg cagagaggtc agcgggtaca
      121 gttctttgtc gatcggctcc tgtttttgcg gtggcgtggt ggtttcaaca ccagaacgga
      181 aagattcttc cggcggtgcg gtcggttccg gacgagactg cagatcttct tcgctaatca
      241 gtttctgttc ggtgcagcca cgaatcttct caacgaacgc cttggccttt tccgccactt
      301 ccaccggggt gcctttcact aacgcgctgc ccacgcccac ggccagcacg cccgctttga
      361 accattcgca cacattgtcc agattaacac cgccggttgg cacaaatttc acattcggaa
      421 acggaccttt catcgcttta acaaactgcg gacccaccac ctcacccgga aacagtttta
      481 agatggtatg gcccagtttc atggctttca ccagttcggt cggggtcata acgcctggca
      541 tatagaacac acccttctcc ttgcaaaatt ggctaatttc ctcgtccagg tgcggactca
      601 caatgaattc ggcgccactt tccaccgctt tacggcactg ctccacgctg gtcacggtgc
      661 ccgcaccaat aatcgcgccc atttccttca gaaagctcag ttccttaatc acggtatccg
      721 catccggcac ggtaaaggta atttcaatca gatgaacgcc gcccaggaaa accgccagcg
      781 ctttcttttt cgcttcttc
//
